Topoisomerase II alpha Antibody / TOP2A

NSJ Bioreagents
Product Code: NSJ-V9310
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-V9310-20UG20ug£264.00
Quantity:
NSJ-V9310-100UG100ug£534.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Recombinant Antibody
Antibody Clone: TOP2A/6570R
Regulatory Status: RUO
Target Species: Human
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Aliquot the recombinant Topoisomerase II alpha antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

1 / 5
IHC staining of FFPE human lymph node tissue with recombinant Topoisomerase II alpha antibody (clone TOP2A/6570R). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
2 / 5
IHC staining of FFPE human tonsil tissue with recombinant Topoisomerase II alpha antibody (clone TOP2A/6570R). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
3 / 5
Negative control: IHC staining of FFPE human brain tissue using recombinant Topoisomerase II alpha antibody (clone TOP2A/6570R) at 2ug/ml in PBS for 30min RT. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
4 / 5
Western blot testing of human HeLa and Jurkat cell lysates using recombinant Topoisomerase II alpha antibody (clone TOP2A/6570R). Predicted molecular weight ~174 kDa.
5 / 5
SDS-PAGE analysis of purified, BSA-free recombinant Topoisomerase II alpha antibody (clone TOP2A/6570R) as confirmation of integrity and purity.

IHC staining of FFPE human lymph node tissue with recombinant Topoisomerase II alpha antibody (clone TOP2A/6570R). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human tonsil tissue with recombinant Topoisomerase II alpha antibody (clone TOP2A/6570R). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
Negative control: IHC staining of FFPE human brain tissue using recombinant Topoisomerase II alpha antibody (clone TOP2A/6570R) at 2ug/ml in PBS for 30min RT. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
Western blot testing of human HeLa and Jurkat cell lysates using recombinant Topoisomerase II alpha antibody (clone TOP2A/6570R). Predicted molecular weight ~174 kDa.
SDS-PAGE analysis of purified, BSA-free recombinant Topoisomerase II alpha antibody (clone TOP2A/6570R) as confirmation of integrity and purity.

Further Information

Application Details :
Western blot: 2-4ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml
Application Note:
Optimal dilution of the recombinant Topoisomerase II alpha antibody should be determined by the researcher.
Description:
The topoisomerase IIa is the predominant mechanism of resistance to several chemotherapeutic agents.
Format :
Purified
Formulation :
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Immunogen:
A portion of amino acids 1352-1493 was used as the immunogen for the recombinant Topoisomerase II alpha antibody.
Limitation:
This recombinant Topoisomerase II alpha antibody is available for research use only.
Localization:
Nuclear
Purity:
Protein A/G affinity
Species Reactivity :
Human
Uniprot #:
P11388