HSP60 Antibody

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NSJ Bioreagents
Product Code: NSJ-V9314
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-V9314-20UG20ug£264.00
Quantity:
NSJ-V9314-100UG100ug£534.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG, kappa
Antibody Clonality: Recombinant Antibody
Antibody Clone: HSPD1/6498R
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Aliquot the HSP60/GROEL antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

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Western blot testing of human 1) Hep2 and 2) HeLa cell lysate using HSP60/GROEL antibody (clone HSPD1/6498R). Predicted molecular weight: ~60 kDa.
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Western blot testing of human Hep2 cell lysate using HSP60/GROEL antibody (clone HSPD1/6498R). Predicted molecular weight: ~60 kDa.
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IHC staining of FFPE human liver tissue with HSP60/GROEL antibody (clone HSPD1/6498R). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
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Western blot testing of human 1) Hep2 and 2) HeLa cell lysate using HSP60/GROEL antibody (clone HSPD1/6498R). Predicted molecular weight: ~60 kDa.
Western blot testing of human Hep2 cell lysate using HSP60/GROEL antibody (clone HSPD1/6498R). Predicted molecular weight: ~60 kDa.
IHC staining of FFPE human liver tissue with HSP60/GROEL antibody (clone HSPD1/6498R). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

Documents

Further Information

Application Details :
Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml
Application Note:
Optimal dilution of the HSP60/GROEL antibody should be determined by the researcher.
Description:
Recognizes a 60kDa protein, identified as the heat shock protein 60 (hsp60). Its epitope is localized between aa 383-447 of human hsp60. A wide variety of environmental and pathophysiological stressful conditions trigger the synthesis of a family of proteins known as heat shock proteins (hsp s), more appropriately called as stress response proteins (srp s). hsp60 is a potential antigen in a number of autoimmune diseases. In human arthritis and in experimentally induced arthritis in animals, disease development coincides with the development of immune reactivity directed against not only bacterial hsp60, but also against its mammalian homolog. Clone HSPD1/6498R, unlike clone LK2, recognizes only the mammalian (not bacterial) hsp60 and is useful in distinguishing hsp60 from mammals and bacteria.
Format :
Purified
Formulation :
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Immunogen:
Recombinant human full-length HSP60 protein was used as the immunogen for the HSP60/GROEL antibody. Its epitope is localized between amino acids 383-447 of human hsp60.
Limitation:
This HSP60/GROEL antibody is available for research use only.
Localization:
Cytoplasm
Purity:
Protein A/G affinity
Species Reactivity :
Human, Mouse, Rat
Uniprot #:
P10809