KRT8 Antibody / Cytokeratin 8

NSJ Bioreagents
Product Code: NSJ-V9325
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-V9325-20UG20ug£264.00
Quantity:
NSJ-V9325-100UG100ug£534.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Recombinant Antibody
Antibody Clone: KRT8/6472R
Regulatory Status: RUO
Target Species: Human
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Aliquot the recombinant Keratin 8 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

1 / 3
IHC staining of FFPE human colon tissue with recombinant Keratin 8 antibody (clone KRT8/6472R). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
2 / 3
Negative control: IHC staining of FFPE human brain tissue using recombinant Keratin 8 antibody (clone KRT8/6472R) at 2ug/ml in PBS for 30min RT. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
3 / 3
SDS-PAGE analysis of purified, BSA-free recombinant Keratin 8 antibody (clone KRT8/6472R) as confirmation of integrity and purity.

IHC staining of FFPE human colon tissue with recombinant Keratin 8 antibody (clone KRT8/6472R). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
Negative control: IHC staining of FFPE human brain tissue using recombinant Keratin 8 antibody (clone KRT8/6472R) at 2ug/ml in PBS for 30min RT. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
SDS-PAGE analysis of purified, BSA-free recombinant Keratin 8 antibody (clone KRT8/6472R) as confirmation of integrity and purity.

Further Information

Application Details :
Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml
Application Note:
Optimal dilution of the recombinant Keratin 8 antibody should be determined by the researcher.
Description:
Cytokeratin 8 (also called CK8, Keratin 8 and KRT8) belongs to the type II (or B or basic) subfamily of high molecular weight cytokeratins and exists in combination with cytokeratin 18 (CK18). CK8 is primarily found in the non-squamous epithelia and is present in majority of adenocarcinomas and ductal carcinomas. It is absent in squamous cell carcinomas. Hepatocellular carcinomas are defined by the use of antibodies that recognize only cytokeratin 8 and 18. CK8 exists on several types of normal and neoplastic epithelia, including many ductal and glandular epithelia such as colon, stomach, small intestine, trachea, and esophagus as well as in transitional epithelium.
Format :
Purified
Formulation :
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Immunogen:
Recombinant full-length human KRT8 protein was used as the immunogen for the recombinant Keratin 8 antibody.
Limitation:
This recombinant Keratin 8 antibody is available for research use only.
Localization:
Cytoplasmic
Purity:
Protein A/G affinity
Species Reactivity :
Human
Uniprot #:
P05787