CD47 Antibody / IAP / Integrin Associated Protein

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NSJ Bioreagents
Product Code: NSJ-V9360
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-V9360-20UG20ug£264.00
Quantity:
NSJ-V9360-100UG100ug£534.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Mouse
Antibody Isotype: Mouse IgG1, kappa
Antibody Clonality: Recombinant Antibody
Antibody Clone: rCD47/6589
Regulatory Status: RUO
Target Species: Human
Application: Immunohistochemistry- Paraffin Embedded (IHC-P)
Storage:
Aliquot the recombinant CD47 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

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IHC staining of FFPE human prostate carcinoma tissue with recombinant CD47 antibody (clone rCD47/6589). Negative control inset: PBS instead of primary antibody to control for secondary binding. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
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IHC staining of FFPE human prostate carcinoma tissue with recombinant CD47 antibody (clone rCD47/6589). Negative control inset: PBS instead of primary antibody to control for secondary binding. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

Documents

Further Information

Application Details :
Immunohistochemistry (FFPE): 1-2ug/ml
Application Note:
Optimal dilution of the recombinant CD47 antibody should be determined by the researcher.
Description:
This antibody reacts with Ig domain of CD47 protein. It has been shown to inhibit polymorphonuclear neutrophil (PMN) transmigration across cell monolayers and matrix. CD47, originally named integrin-associated protein (IAP), is a 50kDa protein containing five membrane-spanning sequences and a short cytoplasmic tail. CD47 plays a role in both cell adhesion by acting as an adhesion receptor for THBS1 on platelets, and in the modulation of integrins. It is important in memory formation and synaptic plasticity in the hippocampus. CD47 may play a role in membrane transport and/or integrin dependent signal transduction.
Format :
Purified
Formulation :
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Immunogen:
Recombinant full-length human protein was used as the immunogen for the recombinant CD47 antibody.
Limitation:
This recombinant CD47 antibody is available for research use only.
Localization:
Cell Surface
Purity:
Protein A/G affinity
Species Reactivity :
Human
Uniprot #:
Q08722