Estrogen Receptor alpha Antibody

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NSJ Bioreagents
Product Code: NSJ-V9392
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-V9392-20UG20ug£264.00
Quantity:
NSJ-V9392-100UG100ug£534.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG, kappa
Antibody Clonality: Recombinant Antibody
Antibody Clone: ESR1/4039R
Regulatory Status: RUO
Target Species: Human
Application: Immunohistochemistry- Paraffin Embedded (IHC-P)
Storage:
Aliquot the recombinant ERa antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

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IHC staining of FFPE human breast carcinoma tissue with recombinant ERa antibody (clone ESR1/4039R). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
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IHC staining of FFPE human breast carcinoma tissue with recombinant ERa antibody (clone ESR1/4039R). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
3 / 3
SDS-PAGE analysis of purified, BSA-free recombinant ERa antibody (clone ESR1/4039R) as confirmation of integrity and purity.
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IHC staining of FFPE human breast carcinoma tissue with recombinant ERa antibody (clone ESR1/4039R). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human breast carcinoma tissue with recombinant ERa antibody (clone ESR1/4039R). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
SDS-PAGE analysis of purified, BSA-free recombinant ERa antibody (clone ESR1/4039R) as confirmation of integrity and purity.

Documents

Further Information

Application Details :
Immunohistochemistry (FFPE): 1-2ug/ml
Application Note:
Optimal dilution of the recombinant ERa antibody should be determined by the researcher.
Description:
The ER gene consists of more than 140kb of genomic DNA divided into 8 exons, being translated into a protein with six functionally discrete domains, labeled A through F. This antibody strongly stains the nucleus of epithelial cells in breast carcinomas. The ER is an important regulator of growth and differentiation in the mammary gland. Presence of ER in breast tumors indicates an increased likelihood of response to antiestrogen (e.g. tamoxifen) therapy.
Format :
Purified
Formulation :
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Immunogen:
A recombinant protein fragment from the C-terminal region was used as the immunogen for the recombinant ERa antibody.
Limitation:
This recombinant ERa antibody is available for research use only.
Localization:
Nucleus
Purity:
Protein A/G affinity
Species Reactivity :
Human
Uniprot #:
P03372