AKR1B1 Antibody / Aldose reductase

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NSJ Bioreagents
Product Code: NSJ-V9399
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-V9399-20UG20ug£264.00
Quantity:
NSJ-V9399-100UG100ug£534.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG, kappa
Antibody Clonality: Recombinant Antibody
Antibody Clone: AKR1B1/7010R
Regulatory Status: RUO
Target Species: Human
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Aliquot the recombinant Aldose reductase antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

1 / 3
IHC staining of FFPE human brain tissue with recombinant Aldose reductase antibody (clone AKR1B1/7010R) at 2ug/ml in PBS for 30 min RT. Negative control inset: PBS used instead of primary antibody to control for secondary Ab binding. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
2 / 3
IHC staining of FFPE human kidney tissue with recombinant Aldose reductase antibody (clone AKR1B1/7009R). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
3 / 3
SDS-PAGE analysis of purified, BSA-free recombinant Aldose reductase antibody (AKR1B1/7010R) as confirmation of integrity and purity.
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IHC staining of FFPE human brain tissue with recombinant Aldose reductase antibody (clone AKR1B1/7010R) at 2ug/ml in PBS for 30 min RT. Negative control inset: PBS used instead of primary antibody to control for secondary Ab binding. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human kidney tissue with recombinant Aldose reductase antibody (clone AKR1B1/7009R). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
SDS-PAGE analysis of purified, BSA-free recombinant Aldose reductase antibody (AKR1B1/7010R) as confirmation of integrity and purity.

Documents

Further Information

Application Details :
Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 2-4ug/ml
Application Note:
Optimal dilution of the recombinant Aldose reductase antibody should be determined by the researcher.
Description:
AKR1B1, also designated as aldose reductase, is a member of the aldo/keto reductase superfamily, which consists of more than 40 known enzymes and proteins. This protein catalyzes the reduction of a number of aldehydes, including the aldehyde form of glucose, and is thereby implicated in the development of diabetic complications by catalyzing the reduction of glucose to sorbitol.
Format :
Purified
Formulation :
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Immunogen:
Recombinant human full-length AKR1B1 protein was used as the immunogen for the recombinant Aldose reductase antibody.
Limitation:
This recombinant Aldose reductase antibody is available for research use only.
Localization:
Cytoplasm
Purity:
Protein A/G affinity
Species Reactivity :
Human
Uniprot #:
P15121