OVOL2 Antibody

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NSJ Bioreagents
Product Code: NSJ-V8773
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-V8773-20UG20ug£264.00
Quantity:
NSJ-V8773-100UG100ug£534.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Mouse
Antibody Isotype: Mouse IgG1
Antibody Clonality: Monoclonal
Antibody Clone: PCRP-OVOL2-2A1
Regulatory Status: RUO
Target Species: Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
Storage:
Aliquot the OVOL2 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

1 / 4
Immunofluorescent staining of PFA-fixed human HeLa cells using OVOL2 antibody (green, clone PCRP-OVOL2-2A1) and phalloidin (red).
2 / 4
FACS staining of PFA-fixed human HeLa cells with OVOL2 antibody (blue, clone PCRP-OVOL2-2A1), and unstained cells (red).
3 / 4
SDS-PAGE analysis of purified, BSA-free OVOL2 antibody (PCRP-OVOL2-2A1) as confirmation of integrity and purity.
4 / 4
Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using OVOL2 antibody (clone PCRP-OVOL2-2A1). These results demonstrate the foremost specificity of the PCRP-OVOL2-2A1 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.
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Immunofluorescent staining of PFA-fixed human HeLa cells using OVOL2 antibody (green, clone PCRP-OVOL2-2A1) and phalloidin (red).
FACS staining of PFA-fixed human HeLa cells with OVOL2 antibody (blue, clone PCRP-OVOL2-2A1), and unstained cells (red).
SDS-PAGE analysis of purified, BSA-free OVOL2 antibody (PCRP-OVOL2-2A1) as confirmation of integrity and purity.
Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using OVOL2 antibody (clone PCRP-OVOL2-2A1). These results demonstrate the foremost specificity of the PCRP-OVOL2-2A1 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.

Documents

Further Information

Application Details :
Flow cytometry: 1-2ug/million cells,Immunofluorescence: 1-2ug/ml
Application Note:
Optimal dilution of the OVOL2 antibody should be determined by the researcher.
Description:
Transcription factor Ovo-like 2 is a zinc-finger transcription repressor factor that suppresses cell cycling and terminal differentiation of keratinocytes by directly repressing MYC and NOTCH1. (UniProt)
Format :
Purified
Formulation :
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Immunogen:
Recombinant full-length human OVOL2 protein was used as the immunogen for the OVOL2 antibody.
Limitation:
This OVOL2 antibody is available for research use only.
Localization:
Nucleus, cytoplasm
Microarray Validated?:
Yes
Purity:
Protein A/G affinity
Species Reactivity :
Human
Uniprot #:
Q9BRP0