UBR2 Antibody

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NSJ Bioreagents
Product Code: NSJ-V8778
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-V8778-20UG20ug£264.00
Quantity:
NSJ-V8778-100UG100ug£534.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Mouse
Antibody Isotype: Mouse IgG2a
Antibody Clonality: Monoclonal
Antibody Clone: PCRP-UBR2-1D12
Regulatory Status: RUO
Target Species: Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Immunoprecipitation (IP)
  • Western Blot (WB)
Storage:
Aliquot the UBR2 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

1 / 5
IHC staining of FFPE human thyroid tissue with UBR2 antibody (clone PCRP-UBR2-1D12) at 2ug/ml. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
2 / 5
FACS staining of PFA-fixed human HeLa cells with UBR2 antibody (blue, clone PCRP-UBR2-1D12) and isotype control (red).
3 / 5
SDS-PAGE analysis of purified, BSA-free UBR2 antibody (PCRP-UBR2-1D12) as confirmation of integrity and purity.
4 / 5
Immunofluorescent staining of PFA-fixed human HeLa cells using UBR2 antibody (green, clone PCRP-UBR2-1D12) and phalloidin (red).
5 / 5
Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using UBR2 antibody (clone PCRP-UBR2-1D12). These results demonstrate the foremost specificity of the PCRP-UBR2-1D12 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.
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IHC staining of FFPE human thyroid tissue with UBR2 antibody (clone PCRP-UBR2-1D12) at 2ug/ml. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
FACS staining of PFA-fixed human HeLa cells with UBR2 antibody (blue, clone PCRP-UBR2-1D12) and isotype control (red).
SDS-PAGE analysis of purified, BSA-free UBR2 antibody (PCRP-UBR2-1D12) as confirmation of integrity and purity.
Immunofluorescent staining of PFA-fixed human HeLa cells using UBR2 antibody (green, clone PCRP-UBR2-1D12) and phalloidin (red).
Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using UBR2 antibody (clone PCRP-UBR2-1D12). These results demonstrate the foremost specificity of the PCRP-UBR2-1D12 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.

Documents

Further Information

Application Details :
Immunoprecipitation: 1-2ug per 100-500ug of total protein (1ml of cell lysate),Flow cytometry: 1-2ug/million cells,Immunofluorescence: 1-2ug/ml,Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml
Application Note:
Optimal dilution of the UBR2 antibody should be determined by the researcher.
Description:
E3 ubiquitin-protein ligase which is a component of the N-end rule pathway. (UniProt)
Format :
Purified
Formulation :
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Immunogen:
Recombinant full-length human UBR2 protein was used as the immunogen for the UBR2 antibody.
Limitation:
This UBR2 antibody is available for research use only.
Localization:
Nucleus, cytoplasm
Microarray Validated?:
Yes
Purity:
Protein A/G affinity
Species Reactivity :
Human
Uniprot #:
Q8IWV8