IRF3 Antibody

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NSJ Bioreagents
Product Code: NSJ-V8820
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-V8820-20UG20ug£264.00
Quantity:
NSJ-V8820-100UG100ug£534.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Mouse
Antibody Isotype: Mouse IgG1, kappa
Antibody Clonality: Monoclonal
Antibody Clone: PCRP-IRF3-3B2
Regulatory Status: RUO
Target Species: Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Western Blot (WB)
Storage:
Aliquot the IRF3 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

1 / 5
Western blot testing of human HeLa cell lysate using IRF3 antibody (clone PCRP-IRF3-3B2). Predicted molecular weight ~47 kDa.
2 / 5
SDS-PAGE analysis of purified, BSA-free IRF3 antibody (PCRP-IRF3-3B2) as confirmation of integrity and purity.
3 / 5
FACS staining of PFA-fixed human HeLa cells with IRF3 antibody (blue, clone PCRP-IRF3-3B2) and isotype control (red).
4 / 5
Immunofluorescent staining of PFA-fixed human HeLa cells using IRF3 antibody (green, clone PCRP-IRF3-3B2) and phalloidin (red).
5 / 5
Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using IRF3 antibody (clone PCRP-IRF3-3B2). These results demonstrate the foremost specificity of the PCRP-IRF3-3B2 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.
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Western blot testing of human HeLa cell lysate using IRF3 antibody (clone PCRP-IRF3-3B2). Predicted molecular weight ~47 kDa.
SDS-PAGE analysis of purified, BSA-free IRF3 antibody (PCRP-IRF3-3B2) as confirmation of integrity and purity.
FACS staining of PFA-fixed human HeLa cells with IRF3 antibody (blue, clone PCRP-IRF3-3B2) and isotype control (red).
Immunofluorescent staining of PFA-fixed human HeLa cells using IRF3 antibody (green, clone PCRP-IRF3-3B2) and phalloidin (red).
Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using IRF3 antibody (clone PCRP-IRF3-3B2). These results demonstrate the foremost specificity of the PCRP-IRF3-3B2 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.

Documents

Further Information

Application Details :
Western blot: 2-4ug/ml,Immunofluorescence: 1-2ug/ml,Flow cytometry: 1-2ug/million cells
Application Note:
Optimal dilution of the IRF3 antibody should be determined by the researcher.
Description:
Interferon regulatory factor 3 is a key transcriptional regulator of type I interferon (IFN)-dependent immune responses which plays a critical role in the innate immune response against DNA and RNA viruses. [UniProt]
Format :
Purified
Formulation :
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Immunogen:
Recombinant full-length human IRF3 protein was used as the immunogen for the IRF3 antibody.
Limitation:
This IRF3 antibody is available for research use only.
Localization:
Cytoplasm, Nucleus
Microarray Validated?:
Yes
Purity:
Protein A/G affinity
Species Reactivity :
Human
Uniprot #:
Q14653