SOX9 Antibody

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NSJ Bioreagents
Product Code: NSJ-V8917
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-V8917-20UG20ug£264.00
Quantity:
NSJ-V8917-100UG100ug£534.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Mouse
Antibody Isotype: Mouse IgG1, kappa
Antibody Clonality: Monoclonal
Antibody Clone: SOX9/2387
Regulatory Status: RUO
Target Species: Human
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Aliquot the SOX9 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

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Western blot testing of human PC3 cell lysate using SOX9 antibody (clone SOX9/2387). Predicted molecular weight: 56-65 kDa.
2 / 7
IHC staining of FFPE human pancreatic tissue with SOX9 antibody (clone SOX9/2387) at 2ug/ml. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
3 / 7
IHC staining of FFPE human pancreatic tissue with SOX9 antibody (clone SOX9/2387) at 2ug/ml. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
4 / 7
IHC staining of FFPE human pancreatic tissue with SOX9 antibody (clone SOX9/2387) at 2ug/ml. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
5 / 7
IHC staining of FFPE human gastric carcinoma tissue with SOX9 antibody (clone SOX9/2387) at 2ug/ml. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
6 / 7
SDS-PAGE analysis of purified, BSA-free SOX9 antibody (clone SOX9/2387) as confirmation of integrity and purity.
7 / 7
Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using SOX9 antibody (clone SOX9/2387). These results demonstrate the foremost specificity of the SOX9/2387 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.
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Western blot testing of human PC3 cell lysate using SOX9 antibody (clone SOX9/2387). Predicted molecular weight: 56-65 kDa.
IHC staining of FFPE human pancreatic tissue with SOX9 antibody (clone SOX9/2387) at 2ug/ml. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human pancreatic tissue with SOX9 antibody (clone SOX9/2387) at 2ug/ml. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human pancreatic tissue with SOX9 antibody (clone SOX9/2387) at 2ug/ml. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human gastric carcinoma tissue with SOX9 antibody (clone SOX9/2387) at 2ug/ml. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
SDS-PAGE analysis of purified, BSA-free SOX9 antibody (clone SOX9/2387) as confirmation of integrity and purity.
Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using SOX9 antibody (clone SOX9/2387). These results demonstrate the foremost specificity of the SOX9/2387 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.

Documents

Further Information

Application Details :
Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml
Application Note:
Optimal dilution of the SOX9 antibody should be determined by the researcher.
Description:
Sox genes comprise a family of genes that are related to the mammalian sex-determining gene SRY. These genes similarly contain sequences that encode for the HMG-box domain, which is responsible for the sequence-specific DNA-binding activity. Sox genes encode putative transcriptional regulators implicated in the decision of cell fates during development and the control of diverse developmental processes. SOX9 plays an important role in the normal skeletal development. It may regulate the expression of other genes involved in chondrogenesis by acting as a transcription factor for these genes.
Format :
Purified
Formulation :
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Immunogen:
A portion of amino acids 393-508 was used as the immunogen for the SOX9 antibody.
Limitation:
This SOX9 antibody is available for research use only.
Localization:
Nucleus
Microarray Validated?:
Yes
Purity:
Protein A/G affinity
Species Reactivity :
Human
Uniprot #:
P48436