FOXB1 Antibody

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NSJ Bioreagents
Product Code: NSJ-V8922
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-V8922-20UG20ug£264.00
Quantity:
NSJ-V8922-100UG100ug£534.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Mouse
Antibody Isotype: Mouse IgG1
Antibody Clonality: Monoclonal
Antibody Clone: PCRP-FOXB1-1B7
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
Storage:
Aliquot the FOXB1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

1 / 4
Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using FOXB1 antibody (clone PCRP-FOXB1-1B7). These results demonstrate the foremost specificity of the PCRP-FOXB1-1B7 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.
2 / 4
FACS staining of PFA-fixed human HeLa cells with FOXB1 antibody (blue, clone PCRP-FOXB1-1B7) and isotype control (red).
3 / 4
Immunofluorescent staining of PFA-fixed human HeLa cells using FOXB1 antibody (green, clone PCRP-FOXB1-1B7) and phalloidin (red).
4 / 4
SDS-PAGE analysis of purified, BSA-free FOXB1 antibody (clone PCRP-FOXB1-1B7) as confirmation of integrity and purity.
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Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using FOXB1 antibody (clone PCRP-FOXB1-1B7). These results demonstrate the foremost specificity of the PCRP-FOXB1-1B7 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.
FACS staining of PFA-fixed human HeLa cells with FOXB1 antibody (blue, clone PCRP-FOXB1-1B7) and isotype control (red).
Immunofluorescent staining of PFA-fixed human HeLa cells using FOXB1 antibody (green, clone PCRP-FOXB1-1B7) and phalloidin (red).
SDS-PAGE analysis of purified, BSA-free FOXB1 antibody (clone PCRP-FOXB1-1B7) as confirmation of integrity and purity.

Documents

Further Information

Application Details :
ELISA (order BSA-free format for coating),Flow cytometry: 1-2ug/million cells,Immunofluorescence: 1-2ug/ml
Application Note:
Optimal dilution of the FOXB1 antibody should be determined by the researcher.
Description:
The Forkhead-box (FOX) genes comprise a superfamily of at least 43 members that encode proteins which are involved in transcriptional regulation and may be associated with the pathogenesis of various cancers. FOXB1 (forkhead box B1), also known as FKH5 or HFKH-5, and FOXB2 (forkhead box B2) are members of the FOX family and each contain one forkhead DNA-binding domain. Both FOXB1 and FOXB2 localize to the nucleus where they are thought to function as transcription factors that can bind to DNA via their forkhead domains. In mice, defects in the gene encoding FOXB1 are associated with retarded development of the central nervous system (CNS), suggesting that FOXB1 may play a role in CNS organization and function.
Format :
Purified
Formulation :
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Immunogen:
Recombinant full-length human FOXB1 protein was used as the immunogen for the FOXB1 antibody.
Limitation:
This FOXB1 antibody is available for research use only.
Localization:
Nucleus
Microarray Validated?:
Yes
Purity:
Protein A/G affinity
Species Reactivity :
Human
Uniprot #:
Q99853