SOX10 Antibody

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NSJ Bioreagents
Product Code: NSJ-V8947
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-V8947-20UG20ug£264.00
Quantity:
NSJ-V8947-100UG100ug£534.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Mouse
Antibody Isotype: Mouse IgG2b
Antibody Clonality: Monoclonal
Antibody Clone: PCRP-SOX10-1D8
Regulatory Status: RUO
Target Species: Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
Storage:
Aliquot the SOX10 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

1 / 4
FACS staining of PFA-fixed human HeLa cells with SOX10 antibody (blue, clone PCRP-SOX10-1D8), and unstained cells (red).
2 / 4
Immunofluorescent staining of PFA-fixed human HeLa cells using SOX10 antibody (green, clone PCRP-SOX10-1D8) and phalloidin (red).
3 / 4
Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using SOX10 antibody (clone PCRP-SOX10-1D8). These results demonstrate the foremost specificity of the PCRP-SOX10-1D8 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.
4 / 4
SDS-PAGE analysis of purified, BSA-free SOX10 antibody (clone PCRP-SOX10-1D8) as confirmation of integrity and purity.
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FACS staining of PFA-fixed human HeLa cells with SOX10 antibody (blue, clone PCRP-SOX10-1D8), and unstained cells (red).
Immunofluorescent staining of PFA-fixed human HeLa cells using SOX10 antibody (green, clone PCRP-SOX10-1D8) and phalloidin (red).
Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using SOX10 antibody (clone PCRP-SOX10-1D8). These results demonstrate the foremost specificity of the PCRP-SOX10-1D8 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.
SDS-PAGE analysis of purified, BSA-free SOX10 antibody (clone PCRP-SOX10-1D8) as confirmation of integrity and purity.

Documents

Further Information

Application Details :
Flow cytometry: 1-2ug/million cells,Immunofluorescence: 1-2ug/ml
Application Note:
Optimal dilution of the SOX10 antibody should be determined by the researcher.
Description:
This MAb is highly specific and does not cross-react with other members of the SOX-family. SOX genes comprise a family of genes that are related to the mammalian sex-determining gene SRY. These genes similarly contain sequences that encode for the HMG-box domain, which is responsible for the sequence-specific DNA-binding activity. SOX10 is a sensitive marker of melanoma, including conventional, spindled, and desmoplastic subtypes. Expressed by metastatic melanomas and nodal capsular nevus in sentinel lymph nodes, but not by other lymph node components such as dendritic cells, which usually express S100 protein. Often used melanoma markers, such as anti-HMB45 and anti-MelanA, are poorly expressed in desmoplastic melanomas while SOX10 is moderately-to-strongly expressed in desmoplastic melanomas.
Format :
Purified
Formulation :
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Immunogen:
A portion of amino acids 90-179 was used as the immunogen for the SOX10 antibody.
Limitation:
This SOX10 antibody is available for research use only.
Localization:
Nuclear
Microarray Validated?:
Yes
Purity:
Protein A/G affinity
Species Reactivity :
Human
Uniprot #:
P56693