CSTF2T Antibody / Cleavage stimulation factor subunit 2 tau variant

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NSJ Bioreagents
Product Code: NSJ-V8957
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-V8957-20UG20ug£264.00
Quantity:
NSJ-V8957-100UG100ug£534.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Mouse
Antibody Isotype: Mouse IgG2b, kappa
Antibody Clonality: Monoclonal
Antibody Clone: PCRP-CSTF2T-1A3
Regulatory Status: RUO
Target Species: Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Aliquot the CSTF2T antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

1 / 5
Western blot testing of human 1) T47-D, 2) SK-BR-3 and 3) A375 cell lysate using CSTF2T antibody (clone PCRP-CSTF2T-1A3). Predicted molecular weight ~64 kDa.
2 / 5
IHC staining of FFPE human colon adenocarcinoma tissue with CSTF2T antibody (clone PCRP-CSTF2T-1A3). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
3 / 5
IHC staining of FFPE human testis tissue with CSTF2T antibody (clone PCRP-CSTF2T-1A3). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
4 / 5
SDS-PAGE analysis of purified, BSA-free CSTF2T antibody (PCRP-CSTF2T-1A3) as confirmation of integrity and purity.
5 / 5
Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using CSTF2T antibody (clone PCRP-CSTF2T-1A3). These results demonstrate the foremost specificity of the PCRP-CSTF2T-1A3 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.
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Western blot testing of human 1) T47-D, 2) SK-BR-3 and 3) A375 cell lysate using CSTF2T antibody (clone PCRP-CSTF2T-1A3). Predicted molecular weight ~64 kDa.
IHC staining of FFPE human colon adenocarcinoma tissue with CSTF2T antibody (clone PCRP-CSTF2T-1A3). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human testis tissue with CSTF2T antibody (clone PCRP-CSTF2T-1A3). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
SDS-PAGE analysis of purified, BSA-free CSTF2T antibody (PCRP-CSTF2T-1A3) as confirmation of integrity and purity.
Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using CSTF2T antibody (clone PCRP-CSTF2T-1A3). These results demonstrate the foremost specificity of the PCRP-CSTF2T-1A3 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.

Documents

Further Information

Application Details :
Flow cytometry: 1-2ug/million cells,Immunofluorescence: 1-2ug/ml,Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml
Application Note:
Optimal dilution of the CSTF2T antibody should be determined by the researcher.
Description:
The CSTF2T gene encodes the nuclear protein 'Cleavage stimulation factor subunit 2 tau variant' with an RRM (RNA recognition motif) domain. The protein is a member of the cleavage stimulation factor (CSTF) complex that is involved in the 3-untranslated region of mRNAs.
Format :
Purified
Formulation :
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Immunogen:
Recombinant full-length human CSTF2T protein was used as the immunogen for the CSTF2T antibody.
Limitation:
This CSTF2T antibody is available for research use only.
Localization:
Nucleus
Microarray Validated?:
Yes
Purity:
Protein A/G affinity
Species Reactivity :
Human
Uniprot #:
Q9H0L4