Geminin Antibody / GMNN

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NSJ Bioreagents
Product Code: NSJ-V8963
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-V8963-20UG20ug£264.00
Quantity:
NSJ-V8963-100UG100ug£534.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Mouse
Antibody Isotype: Mouse IgG1, kappa
Antibody Clonality: Monoclonal
Antibody Clone: GMNN/3665
Regulatory Status: RUO
Target Species: Human
Applications:
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
Storage:
Aliquot the GMNN antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

1 / 4
IHC staining of FFPE human spleen tissue with GMNN antibody (clone GMNN/3665). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
2 / 4
IHC staining of FFPE human testis tissue with GMNN antibody (clone GMNN/3665). Negative control inset: PBS instead of primary antibody to control for secondary binding. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
3 / 4
Immunofluorescent staining of PFA-fixed human MCF-7 cells using GMNN antibody (clone GMNN/3665).
4 / 4
Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using GMNN antibody (clone GMNN/3665). These results demonstrate the foremost specificity of the GMNN/3665 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.
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IHC staining of FFPE human spleen tissue with GMNN antibody (clone GMNN/3665). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human testis tissue with GMNN antibody (clone GMNN/3665). Negative control inset: PBS instead of primary antibody to control for secondary binding. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
Immunofluorescent staining of PFA-fixed human MCF-7 cells using GMNN antibody (clone GMNN/3665).
Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using GMNN antibody (clone GMNN/3665). These results demonstrate the foremost specificity of the GMNN/3665 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.

Documents

Further Information

Application Details :
Immunofluorescence: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml
Application Note:
Optimal dilution of the GMNN antibody should be determined by the researcher.
Description:
Geminin is a nuclear protein that regulates the initiation of DNA replication during the cell cycle.
Format :
Purified
Formulation :
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Immunogen:
A recombinant protein fragment was used as the immunogen for the GMNN antibody.
Limitation:
This GMNN antibody is available for research use only.
Localization:
Nuclear, cytoplasmic
Microarray Validated?:
Yes
Purity:
Protein A/G affinity
Species Reactivity :
Human
Uniprot #:
O75496