CBFB Antibody

Contact us
NSJ Bioreagents
Product Code: NSJ-V8976
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-V8976-20UG20ug£264.00
Quantity:
NSJ-V8976-100UG100ug£534.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Mouse
Antibody Isotype: Mouse IgG2b
Antibody Clonality: Monoclonal
Antibody Clone: PCRP-CBFB-1F6
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Western Blot (WB)
Storage:
Aliquot the CBFB antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

1 / 5
Immunofluorescent staining of PFA-fixed human HeLa cells using CBFB antibody (green, clone PCRP-CBFB-1F6) and phalloidin (red).
2 / 5
FACS staining of PFA-fixed human HeLa cells with CBFB antibody (blue, clone PCRP-CBFB-1F6) and isotype control (red).
3 / 5
Western blot testing of human HeLa cell lysate using CBFB antibody (clone PCRP-CBFB-1F6). Predicted molecular weight ~22 kDa.
4 / 5
SDS-PAGE analysis of purified, BSA-free CBFB antibody (clone PCRP-CBFB-1F6) as confirmation of integrity and purity.
5 / 5
Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using CBFB antibody (clone PCRP-CBFB-1F6). These results demonstrate the foremost specificity of the PCRP-CBFB-1F6 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.
❮❯

Immunofluorescent staining of PFA-fixed human HeLa cells using CBFB antibody (green, clone PCRP-CBFB-1F6) and phalloidin (red).
FACS staining of PFA-fixed human HeLa cells with CBFB antibody (blue, clone PCRP-CBFB-1F6) and isotype control (red).
Western blot testing of human HeLa cell lysate using CBFB antibody (clone PCRP-CBFB-1F6). Predicted molecular weight ~22 kDa.
SDS-PAGE analysis of purified, BSA-free CBFB antibody (clone PCRP-CBFB-1F6) as confirmation of integrity and purity.
Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using CBFB antibody (clone PCRP-CBFB-1F6). These results demonstrate the foremost specificity of the PCRP-CBFB-1F6 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.

Documents

Further Information

Application Details :
ELISA (order BSA-free format for coating),Western blot: 1-2ug/ml,Flow cytometry: 1-2ug/million cells,Immunofluorescence: 1-2ug/ml
Application Note:
Optimal dilution of the CBFB antibody should be determined by the researcher.
Description:
The transcription factor polyomavirus enhancer binding protein 2 (PEBP2), also designated Osf2 (osteoblast-specific transcription factor), CBFA1 (core binding factor) and AML3 (acute myeloid leukemia), is composed of two subunits, /CBFB-SMMHC, that is associated with human acute myeloid leukemia.
Format :
Purified
Formulation :
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Immunogen:
Recombinant full-length human CBFB protein was used as the immunogen for the CBFB antibody.
Limitation:
This CBFB antibody is available for research use only.
Localization:
Nucleus, Nucleus, Cytoplasm
Microarray Validated?:
Yes
Purity:
Protein A/G affinity
Species Reactivity :
Human
Uniprot #:
Q13951