NACC1 Antibody / Nac1 / BTBD14B

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NSJ Bioreagents
Product Code: NSJ-V9183
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-V9183-20UG20ug£264.00
Quantity:
NSJ-V9183-100UG100ug£534.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Mouse
Antibody Isotype: Mouse IgG2a
Antibody Clonality: Monoclonal
Antibody Clone: PCRP-NACC1-1A8
Regulatory Status: RUO
Target Species: Human
Application: Fluorescence-activated cell sorting (FACS)
Storage:
Aliquot the NACC1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

1 / 3
Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using NACC1 antibody (clone PCRP-NACC1-1A8). These results demonstrate the foremost specificity of the PCRP-NACC1-1A8 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.
2 / 3
FACS staining of PFA-fixed human HeLa cells with NACC1 antibody (blue, clone PCRP-NACC1-1A8), and unstained cells (red).
3 / 3
SDS-PAGE analysis of purified, BSA-free NACC1 antibody (clone PCRP-NACC1-1A8) as confirmation of integrity and purity.
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Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using NACC1 antibody (clone PCRP-NACC1-1A8). These results demonstrate the foremost specificity of the PCRP-NACC1-1A8 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.
FACS staining of PFA-fixed human HeLa cells with NACC1 antibody (blue, clone PCRP-NACC1-1A8), and unstained cells (red).
SDS-PAGE analysis of purified, BSA-free NACC1 antibody (clone PCRP-NACC1-1A8) as confirmation of integrity and purity.

Documents

Further Information

Application Details :
Flow cytometry: 1-2ug/million cells
Application Note:
Optimal dilution of the NACC1 antibody should be determined by the researcher.
Description:
The BTB (broad-complex, tramtrack and bric a brac) domain, also known as the POZ (poxvirus and zinc finger) domain, is an N-terminal homodimerization domain that contains multiple copies of kelch repeats and/or C2H2-type zinc fingers. Proteins that contain BTB domains are thought to be involved in transcriptional regulation via control of chromatin structure and function. BTBD14B (BTB/POZ domain-containing protein 14B), also known as NACC1 (nucleus accumbens associated 1), BEND8 or NAC1, is a 527 amino acid protein that localizes to both the nucleus and the cytoplasm and contains one BTB (POZ) domain. Existing as a homo-oligomer that interacts with HDAC3 and HDAC4, BTBD14B functions as a transcriptional repressor that influences the transcriptional activity of CRIF1 and is required for proteasome recruitment to the nucleus and cytoplasm in dendritic spines. BTBD14B is overexpressed in multiple carcinomas, suggesting a role in tumor development and metastasis.
Format :
Purified
Formulation :
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Immunogen:
Recombinant full-length human NACC1 protein was used as the immunogen for the NACC1 antibody.
Limitation:
This NACC1 antibody is available for research use only.
Localization:
Nucleus, Cytoplasm
Microarray Validated?:
Yes
Purity:
Protein A/G affinity
Species Reactivity :
Human
Uniprot #:
Q96RE7