IRF3 Antibody

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NSJ Bioreagents
Product Code: NSJ-V9201
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-V9201-20UG20ug£264.00
Quantity:
NSJ-V9201-100UG100ug£534.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Mouse
Antibody Isotype: Mouse IgG2b, kappa
Antibody Clonality: Monoclonal
Antibody Clone: PCRP-IRF3-6C8
Regulatory Status: RUO
Target Species: Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Western Blot (WB)
Storage:
Aliquot the IRF3 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

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Immunofluorescent staining of human MCF-7 cells using IRF3 antibody (green, clone PCRP-IRF3-6C8) and phalloidin (red).
2 / 11
Immunofluorescent staining of human K562 cells using IRF3 antibody (green, clone PCRP-IRF3-6C8) and phalloidin (red).
3 / 11
FACS staining of PFA-fixed human MCF-7 cells with IRF3 antibody (blue, clone PCRP-IRF3-6C8), and unstained cells (red).
4 / 11
FACS staining of PFA-fixed human K562 cells with IRF3 antibody (blue, clone PCRP-IRF3-6C8), and unstained cells (red).
5 / 11
FACS staining of PFA-fixed human HeLa cells with IRF3 antibody (blue, clone PCRP-IRF3-6C8), and unstained cells (red).
6 / 11
FACS staining of PFA-fixed human U87 cells with IRF3 antibody (blue, clone PCRP-IRF3-6C8), and unstained cells (red).
7 / 11
FACS staining of PFA-fixed human Raji cells with IRF3 antibody (blue, clone PCRP-IRF3-6C8), and unstained cells (red).
8 / 11
Western blot testing of human HeLa cell lysate using IRF3 antibody (clone PCRP-IRF3-6C8). Predicted molecular weight ~47 kDa.
9 / 11
Western blot testing of human MCF7 cell lysate using IRF3 antibody (clone PCRP-IRF3-6C8). Predicted molecular weight ~47 kDa.
10 / 11
Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using IRF3 antibody (clone PCRP-IRF3-6C8). These results demonstrate the foremost specificity of the PCRP-IRF3-6C8 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.
11 / 11
SDS-PAGE analysis of purified, BSA-free IRF3 antibody (PCRP-IRF3-6C8) as confirmation of integrity and purity.
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Immunofluorescent staining of human MCF-7 cells using IRF3 antibody (green, clone PCRP-IRF3-6C8) and phalloidin (red).
Immunofluorescent staining of human K562 cells using IRF3 antibody (green, clone PCRP-IRF3-6C8) and phalloidin (red).
FACS staining of PFA-fixed human MCF-7 cells with IRF3 antibody (blue, clone PCRP-IRF3-6C8), and unstained cells (red).
FACS staining of PFA-fixed human K562 cells with IRF3 antibody (blue, clone PCRP-IRF3-6C8), and unstained cells (red).
FACS staining of PFA-fixed human HeLa cells with IRF3 antibody (blue, clone PCRP-IRF3-6C8), and unstained cells (red).
FACS staining of PFA-fixed human U87 cells with IRF3 antibody (blue, clone PCRP-IRF3-6C8), and unstained cells (red).
FACS staining of PFA-fixed human Raji cells with IRF3 antibody (blue, clone PCRP-IRF3-6C8), and unstained cells (red).
Western blot testing of human HeLa cell lysate using IRF3 antibody (clone PCRP-IRF3-6C8). Predicted molecular weight ~47 kDa.
Western blot testing of human MCF7 cell lysate using IRF3 antibody (clone PCRP-IRF3-6C8). Predicted molecular weight ~47 kDa.
Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using IRF3 antibody (clone PCRP-IRF3-6C8). These results demonstrate the foremost specificity of the PCRP-IRF3-6C8 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.
SDS-PAGE analysis of purified, BSA-free IRF3 antibody (PCRP-IRF3-6C8) as confirmation of integrity and purity.

Documents

Further Information

Application Details :
Flow cytometry: 1-2ug/million cells,Immunofluorescence: 1-2ug/ml,Western blot: 1-2ug/ml
Application Note:
Optimal dilution of the IRF3 antibody should be determined by the researcher.
Description:
In the absence of viral infection, IRF3 is maintained as a monomer in an autoinhibited state. Phosphorylation by TBK1 and IKBKE disrupts this autoinhibition leading to the liberation of the DNA-binding and dimerization activities and its nuclear localization where it can activate type I IFN and ISG genes. [UniProt]
Format :
Purified
Formulation :
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Immunogen:
Recombinant full-length human IRF3 protein was used as the immunogen for the IRF3 antibody.
Limitation:
This IRF3 antibody is available for research use only.
Localization:
Cytoplasm, Nucleus
Microarray Validated?:
Yes
Purity:
Protein A/G affinity
Species Reactivity :
Human
Uniprot #:
Q14653