NOC4L Antibody

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NSJ Bioreagents
Product Code: NSJ-V9212
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-V9212-20UG20ug£264.00
Quantity:
NSJ-V9212-100UG100ug£534.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Mouse
Antibody Isotype: Mouse IgG1
Antibody Clonality: Monoclonal
Antibody Clone: PCRP-NOC4L-1E3
Regulatory Status: RUO
Target Species: Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
Storage:
Aliquot the NOC4L antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

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Immunofluorescent staining of PFA-fixed human HeLa cells using NOC4L antibody (green, clone PCRP-NOC4L-1E3) and phalloidin (red).
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Immunofluorescent staining of PFA-fixed human HeLa cells NOC4L antibody (green, clone PCRP-NOC4L-1E3) and phalloidin (red).
3 / 5
FACS staining of PFA-fixed human HeLa cells with NOC4L antibody (blue, clone PCRP-NOC4L-1E3), and unstained cells (red).
4 / 5
SDS-PAGE analysis of purified, BSA-free NOC4L antibody (clone PCRP-NOC4L-1E3) as confirmation of integrity and purity.
5 / 5
Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using NOC4L antibody (clone PCRP-NOC4L-1E3). These results demonstrate the foremost specificity of the PCRP-NOC4L-1E3 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.
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Immunofluorescent staining of PFA-fixed human HeLa cells using NOC4L antibody (green, clone PCRP-NOC4L-1E3) and phalloidin (red).
Immunofluorescent staining of PFA-fixed human HeLa cells NOC4L antibody (green, clone PCRP-NOC4L-1E3) and phalloidin (red).
FACS staining of PFA-fixed human HeLa cells with NOC4L antibody (blue, clone PCRP-NOC4L-1E3), and unstained cells (red).
SDS-PAGE analysis of purified, BSA-free NOC4L antibody (clone PCRP-NOC4L-1E3) as confirmation of integrity and purity.
Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using NOC4L antibody (clone PCRP-NOC4L-1E3). These results demonstrate the foremost specificity of the PCRP-NOC4L-1E3 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.

Documents

Further Information

Application Details :
Flow cytometry: 1-2ug/million cells,Immunofluorescence: 1-2ug/ml
Application Note:
Optimal dilution of the NOC4L antibody should be determined by the researcher.
Description:
Treg activation is critical for maintaining self-tolerance, but the translational control of this process is still poorly understood. Zhu et al. report a conserved ribosome biogenesis factor, Noc4L, that regulates mRNAs related to Treg and Tconv activation but does not affect global protein translation. Noc4L regulates translation of mRNAs related to Treg activation. Noc4L plays critical roles in activation of Tregs and Tconvs. Noc4L-mediated ribosome biogenesis is critical in controlling the activation of Tregs and maintaining immune tolerance. [Zhu et al., 2019, Cell Reports 27, 1205 1220.]
Format :
Purified
Formulation :
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Immunogen:
Recombinant full-length human NOC4L protein was used as the immunogen for the NOC4L antibody.
Limitation:
This NOC4L antibody is available for research use only.
Localization:
Nucleus
Microarray Validated?:
Yes
Purity:
Protein A/G affinity
Species Reactivity :
Human
Uniprot #:
Q9BVI4