Transcription factor Spi-C / SPIC

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NSJ Bioreagents
Product Code: NSJ-V9223
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-V9223-20UG20ug£264.00
Quantity:
NSJ-V9223-100UG100ug£534.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Mouse
Antibody Isotype: Mouse IgG2b
Antibody Clonality: Monoclonal
Antibody Clone: PCRP-SPIC-2C5
Regulatory Status: RUO
Target Species: Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
Storage:
Aliquot the SPIC antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

1 / 4
Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using SPIC antibody (clone PCRP-SPIC-2C5). These results demonstrate the foremost specificity of the PCRP-SPIC-2C5 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.
2 / 4
FACS staining of PFA-fixed human HeLa cells with SPIC antibody (blue, clone PCRP-SPIC-2C5), and unstained cells (red).
3 / 4
Immunofluorescent staining of PFA-fixed human HeLa cells using SPIC antibody (green, clone PCRP-SPIC-2C5) and phalloidin (red).
4 / 4
SDS-PAGE analysis of purified, BSA-free SPIC antibody (clone PCRP-SPIC-2C5) as confirmation of integrity and purity.
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Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using SPIC antibody (clone PCRP-SPIC-2C5). These results demonstrate the foremost specificity of the PCRP-SPIC-2C5 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.
FACS staining of PFA-fixed human HeLa cells with SPIC antibody (blue, clone PCRP-SPIC-2C5), and unstained cells (red).
Immunofluorescent staining of PFA-fixed human HeLa cells using SPIC antibody (green, clone PCRP-SPIC-2C5) and phalloidin (red).
SDS-PAGE analysis of purified, BSA-free SPIC antibody (clone PCRP-SPIC-2C5) as confirmation of integrity and purity.

Documents

Further Information

Application Details :
Flow cytometry: 1-2ug/million cells,Immunofluorescence: 1-2ug/ml
Application Note:
Optimal dilution of the SPIC antibody should be determined by the researcher.
Description:
The Ets transcription factor family is comprised of DNA-binding proteins that influence lymphoid development and activity and bind the consensus DNA site GGA(A/T) through a unique winged helix-turn-helix motif known as the Ets domain. Spi-B and Spi-C (also known as SPIC) are closely related Ets family members which share a conserved divergent sequence within the Ets domain that enables their binding to non-canonical AGAA sites. Spi-C is a 248 amino acid protein that localizes to the nucleus and, like other Ets family members, binds DNA as a monomer and plays a role in transcriptional regulation. Addi- tionally, Spi-C is thought to control the development of red pulp macrophages, thereby contributing to iron homeostasis and red blood cell recycling. Human Spi-C shares 65% amino acid identity with its mouse counterpart, suggesting a conserved role between species.
Format :
Purified
Formulation :
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Immunogen:
Recombinant full-length human Transcription factor Spi-C protein was used as the immunogen for the SPIC antibody.
Limitation:
This SPIC antibody is available for research use only.
Localization:
Nucleus
Microarray Validated?:
Yes
Purity:
Protein A/G affinity
Species Reactivity :
Human
Uniprot #:
Q8N5J4