ZBTB46 Antibody / BTBD4 / ZNF340

Contact us
NSJ Bioreagents
Product Code: NSJ-V9224
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-V9224-20UG20ug£264.00
Quantity:
NSJ-V9224-100UG100ug£534.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Mouse
Antibody Isotype: Mouse IgG2a
Antibody Clonality: Monoclonal
Antibody Clone: PCRP-ZBTB46-2B8
Regulatory Status: RUO
Target Species: Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Aliquot the ZBTB46 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

1 / 4
FACS staining of PFA-fixed human HeLa cells with ZBTB46 antibody (blue, clone PCRP-ZBTB46-2B8), and unstained cells (red).
2 / 4
IHC staining of FFPE human tonsil tissue with ZBTB46 antibody (clone PCRP-ZBTB46-2B8). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
3 / 4
Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using ZBTB46 antibody (clone PCRP-ZBTB46-2B8). These results demonstrate the foremost specificity of the PCRP-ZBTB46-2B8 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.
4 / 4
SDS-PAGE analysis of purified, BSA-free ZBTB46 antibody (PCRP-ZBTB46-2B8) as confirmation of integrity and purity.
❮❯

FACS staining of PFA-fixed human HeLa cells with ZBTB46 antibody (blue, clone PCRP-ZBTB46-2B8), and unstained cells (red).
IHC staining of FFPE human tonsil tissue with ZBTB46 antibody (clone PCRP-ZBTB46-2B8). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using ZBTB46 antibody (clone PCRP-ZBTB46-2B8). These results demonstrate the foremost specificity of the PCRP-ZBTB46-2B8 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.
SDS-PAGE analysis of purified, BSA-free ZBTB46 antibody (PCRP-ZBTB46-2B8) as confirmation of integrity and purity.

Documents

Further Information

Application Details :
Flow cytometry: 1-2ug/million cells,Immunofluorescence: 1-2ug/ml,Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml
Application Note:
Optimal dilution of the ZBTB46 antibody should be determined by the researcher.
Description:
BTBD4 (BTB/POZ domain-containing protein 4), also known as zinc finger protein 340 (ZNF340) or zinc finger and BTB domain-containing protein 46 (ZBTB46), is a 589 amino acid protein that contains one BTB/POZ domain. The BTB/POZ domain mediates homomeric and heteromeric POZ-POZ inter- actions and is common to transcriptional regulators involved in chromatin modeling. In several BTB/POZ containing proteins, including BCL-6 and the promyelocytic leukemia zinc-finger (PLZF) oncoprotein, this domain interacts with the SMRT/N-CoR-mSin3A HDAC complex and is directly involved in repressing and silencing gene transcription. When this domain is deleted, as with the oncogenic PLZF-RAR chimera of promyelocytic leukemias, this tran- scriptional repression is attenuated. This suggests that BTBD4 may play a role in transcription regulation.
Format :
Purified
Formulation :
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Immunogen:
Recombinant full-length human Zinc finger and BTB domain-containing protein 46 protein was used as the immunogen for the ZBTB46 antibody.
Limitation:
This ZBTB46 antibody is available for research use only.
Localization:
Nucleus
Microarray Validated?:
Yes
Purity:
Protein A/G affinity
Species Reactivity :
Human
Uniprot #:
Q86UZ6