Th-Pok Antibody / ZBTB7B

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NSJ Bioreagents
Product Code: NSJ-V9232
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-V9232-20UG20ug£264.00
Quantity:
NSJ-V9232-100UG100ug£534.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Mouse
Antibody Isotype: Mouse IgG2b
Antibody Clonality: Monoclonal
Antibody Clone: PCRP-ZBTB7B-1B6
Regulatory Status: RUO
Target Species: Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Western Blot (WB)
Storage:
Aliquot the Th-Pok antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

1 / 4
Immunofluorescent staining of PFA-fixed human HeLa cells using Th-Pok antibody (green, clone PCRP-ZBTB7B-1B6) and phalloidin (red).
2 / 4
FACS staining of PFA-fixed human HeLa cells with Th-Pok antibody (blue, clone PCRP-ZBTB7B-1B6), and unstained cells (red).
3 / 4
Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using Th-Pok antibody (clone PCRP-ZBTB7B-1B6). These results demonstrate the foremost specificity of the PCRP-ZBTB7B-1B6 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.
4 / 4
SDS-PAGE analysis of purified, BSA-free Th-Pok antibody (PCRP-ZBTB7B-1B6) as confirmation of integrity and purity.
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Immunofluorescent staining of PFA-fixed human HeLa cells using Th-Pok antibody (green, clone PCRP-ZBTB7B-1B6) and phalloidin (red).
FACS staining of PFA-fixed human HeLa cells with Th-Pok antibody (blue, clone PCRP-ZBTB7B-1B6), and unstained cells (red).
Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using Th-Pok antibody (clone PCRP-ZBTB7B-1B6). These results demonstrate the foremost specificity of the PCRP-ZBTB7B-1B6 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.
SDS-PAGE analysis of purified, BSA-free Th-Pok antibody (PCRP-ZBTB7B-1B6) as confirmation of integrity and purity.

Documents

Further Information

Application Details :
Flow cytometry: 1-2ug/million cells,Immunofluorescence: 1-2ug/ml,Western blot: 1-2ug/ml
Application Note:
Optimal dilution of the Th-Pok antibody should be determined by the researcher.
Description:
TH-POK (T-helper-inducing POZ/Kr ppel-like factor), also known as zinc finger protein 67 (ZFP67), zinc finger and BTB domain-containing protein 7B or Kr ppel-related zinc finger protein cKrox, is a 539 amino acid protein that contains one BTB (POZ) domain and four C2H2-type zinc fingers. Localized to the nucleus, TH-POK functions primarily as a key regulator of lineage commitment of immature T-cell precursors. Specifically, the presence of TH-POK directs positively selected thymocytes to the CD4 lineage, whereas its absence causes default development to the CD8 lineage. TH-POK also functions as a transcriptional repressor of various other genes, such as COL1A1, COL1A2 and Fibronectin.
Format :
Purified
Formulation :
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Immunogen:
Recombinant full-length human Zinc finger and BTB domain-containing protein 7B protein was used as the immunogen for the Th-Pok antibody.
Limitation:
This Th-Pok antibody is available for research use only.
Localization:
Nucleus
Microarray Validated?:
Yes
Purity:
Protein A/G affinity
Species Reactivity :
Human
Uniprot #:
O15156