THG1 Antibody / RHOXF2

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NSJ Bioreagents
Product Code: NSJ-V9251
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-V9251-20UG20ug£264.00
Quantity:
NSJ-V9251-100UG100ug£534.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Mouse
Antibody Isotype: Mouse IgG2b
Antibody Clonality: Monoclonal
Antibody Clone: PCRP-RHOXF2-1D7
Regulatory Status: RUO
Target Species: Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
Storage:
Aliquot the RHOXF2 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

1 / 4
Immunofluorescent staining of human HeLa cells using RHOXF2 antibody (green, clone PCRP-RHOXF2-1D7) and phalloidin (red).
2 / 4
FACS staining of PFA-fixed human HeLa cells with RHOXF2 antibody (blue, clone PCRP-RHOXF2-1D7), and unstained cells (red).
3 / 4
Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using RHOXF2 antibody (clone PCRP-RHOXF2-1D7). These results demonstrate the foremost specificity of the PCRP-RHOXF2-1D7 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.
4 / 4
SDS-PAGE analysis of purified, BSA-free RHOXF2 antibody (clone PCRP-RHOXF2-1D7) as confirmation of integrity and purity.
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Immunofluorescent staining of human HeLa cells using RHOXF2 antibody (green, clone PCRP-RHOXF2-1D7) and phalloidin (red).
FACS staining of PFA-fixed human HeLa cells with RHOXF2 antibody (blue, clone PCRP-RHOXF2-1D7), and unstained cells (red).
Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using RHOXF2 antibody (clone PCRP-RHOXF2-1D7). These results demonstrate the foremost specificity of the PCRP-RHOXF2-1D7 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.
SDS-PAGE analysis of purified, BSA-free RHOXF2 antibody (clone PCRP-RHOXF2-1D7) as confirmation of integrity and purity.

Documents

Further Information

Application Details :
Flow cytometry: 1-2ug/million cells,Immunofluorescence: 1-2ug/ml
Application Note:
Optimal dilution of the RHOXF2 antibody should be determined by the researcher.
Description:
RHOXF2 (Rhox homeobox family member 2), also known as THG1 (Testis homeobox gene 1) belongs to the paired-like homeobox family, PEPP subfamily. It is a transcription factor that maybe involved in reproductive processes. It modulates expression of target genes encoding proteins involved in processes relevant to spermatogenesis. [UniProt]
Format :
Purified
Formulation :
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Immunogen:
Recombinant full-length human Rhox homeobox family member 2 protein was used as the immunogen for the RHOXF2 antibody.
Limitation:
This RHOXF2 antibody is available for research use only.
Localization:
Nucleus
Microarray Validated?:
Yes
Purity:
Protein A/G affinity
Species Reactivity :
Human
Uniprot #:
Q9BQY4