ZBED1 Antibody / DREF

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NSJ Bioreagents
Product Code: NSJ-V9255
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-V9255-20UG20ug£264.00
Quantity:
NSJ-V9255-100UG100ug£534.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Mouse
Antibody Isotype: Mouse IgG2a
Antibody Clonality: Monoclonal
Antibody Clone: PCRP-ZBED1-1E1
Regulatory Status: RUO
Target Species: Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Western Blot (WB)
Storage:
Aliquot the ZBED1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

1 / 5
Immunofluorescent staining of PFA-fixed human HeLa cells using ZBED1 antibody (green, clone PCRP-ZBED1-1E1) and phalloidin (red).
2 / 5
Immunofluorescent staining of PFA-fixed human MCF-7 cells using ZBED1 antibody (green, clone PCRP-ZBED1-1E1) and phalloidin (red).
3 / 5
FACS staining of PFA-fixed human HeLa cells with ZBED1 antibody (blue, clone PCRP-ZBED1-1E1), and unstained cells (red).
4 / 5
Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using ZBED1 antibody (clone PCRP-ZBED1-1E1). These results demonstrate the foremost specificity of the PCRP-ZBED1-1E1 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.
5 / 5
SDS-PAGE analysis of purified, BSA-free ZBED1 antibody (clone PCRP-ZBED1-1E1) as confirmation of integrity and purity.
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Immunofluorescent staining of PFA-fixed human HeLa cells using ZBED1 antibody (green, clone PCRP-ZBED1-1E1) and phalloidin (red).
Immunofluorescent staining of PFA-fixed human MCF-7 cells using ZBED1 antibody (green, clone PCRP-ZBED1-1E1) and phalloidin (red).
FACS staining of PFA-fixed human HeLa cells with ZBED1 antibody (blue, clone PCRP-ZBED1-1E1), and unstained cells (red).
Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using ZBED1 antibody (clone PCRP-ZBED1-1E1). These results demonstrate the foremost specificity of the PCRP-ZBED1-1E1 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.
SDS-PAGE analysis of purified, BSA-free ZBED1 antibody (clone PCRP-ZBED1-1E1) as confirmation of integrity and purity.

Documents

Further Information

Application Details :
Flow cytometry: 1-2ug/million cells,Immunofluorescence: 1-2ug/ml,Western blot: 1-2ug/ml
Application Note:
Optimal dilution of the ZBED1 antibody should be determined by the researcher.
Description:
ZBED1 (zinc finger BED domain-containing protein 1), also known as ALTE (Ac-like transposable element), DREF or TRAMP, is a 694 amino acid protein that localizes specifically to granular structures within the nucleus. Expressed ubiquitously at low levels and present at higher levels in heart, placenta, spleen and skeletal muscle, ZBED1 is thought to function as a transcription factor that regulates a number of ribosomal protein (RP) encoding genes, thereby playing a role in the cell cycle and in cell proliferation events. ZBED1 contains one BED-type zinc finger and binds specifically to 5 DNA regions found in RP promotors. Additionally, ZBED1 binds strongly to the promotor region of Histone H1 (a protein required for the condensation of nucleosomes into higher order structures), subsequently activating H1 transcription.
Format :
Purified
Formulation :
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Immunogen:
Recombinant full-length human ZBED1 protein was used as the immunogen for the ZBED1 antibody.
Limitation:
This ZBED1 antibody is available for research use only.
Localization:
Nucleus
Microarray Validated?:
Yes
Purity:
Protein A/G affinity
Species Reactivity :
Human
Uniprot #:
O96006