AGO3 Antibody / Argonaute-3 / eIF2C3

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NSJ Bioreagents
Product Code: NSJ-V9391
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-V9391-20UG20ug£264.00
Quantity:
NSJ-V9391-100UG100ug£534.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Mouse
Antibody Isotype: Mouse IgG1, kappa
Antibody Clonality: Monoclonal
Antibody Clone: PCRP-AGO3-1C5
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
Storage:
Aliquot the AGO3 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

1 / 4
Immunofluorescent staining of PFA-fixed human HeLa cells. Cytoplasmic P-bodies and nucleoplasm show staining with AGO3 antibody (green, clone PCRP-AGO3-1C5), and phalloidin counterstain (red).
2 / 4
FACS staining of PFA-fixed human HeLa cells with AGO3 antibody (blue, clone PCRP-AGO3-1C5) and isotype control (red).
3 / 4
SDS-PAGE analysis of purified, BSA-free AGO3 antibody (clone PCRP-AGO3-1C5) as confirmation of integrity and purity.
4 / 4
Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using AGO3 antibody (clone PCRP-AGO3-1C5). These results demonstrate the foremost specificity of the PCRP-AGO3-1C5 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.
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Immunofluorescent staining of PFA-fixed human HeLa cells. Cytoplasmic P-bodies and nucleoplasm show staining with AGO3 antibody (green, clone PCRP-AGO3-1C5), and phalloidin counterstain (red).
FACS staining of PFA-fixed human HeLa cells with AGO3 antibody (blue, clone PCRP-AGO3-1C5) and isotype control (red).
SDS-PAGE analysis of purified, BSA-free AGO3 antibody (clone PCRP-AGO3-1C5) as confirmation of integrity and purity.
Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using AGO3 antibody (clone PCRP-AGO3-1C5). These results demonstrate the foremost specificity of the PCRP-AGO3-1C5 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.

Documents

Further Information

Application Details :
ELISA (order BSA-free format for coating),Flow cytometry: 1-2ug/million cells,Immunofluorescence: 1-2ug/ml
Application Note:
Optimal dilution of the AGO3 antibody should be determined by the researcher.
Description:
This antigen is involved in binding and translation repression of short RNAs. This antigen is also known as Argonaute RISC catalytic component 3. Required for RNA-mediated gene silencing (RNAi). Binds to short RNAs such as microRNAs (miRNAs) and represses the translation of mRNAs which are complementary to them. Proposed to be involved in stabilization of small RNA derivates (siRNA) derived from processed RNA polymerase III-transcribed Alu repeats containing a DR2 retinoic acid response element (RARE) in stem cells and in the subsequent siRNA-dependent degradation of a subset of RNA polymerase II-transcribed coding mRNAs by recruiting a mRNA decapping complex involving EDC4. Possesses RNA slicer activity but only on select RNAs bearing 5-flanking sequences to the region of guide-target complementarity.
Format :
Purified
Formulation :
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Immunogen:
Recombinant full-length human AGO3 protein was used as the immunogen for the AGO3 antibody.
Limitation:
This AGO3 antibody is available for research use only.
Localization:
Cytoplasm, Nucleus
Microarray Validated?:
Yes
Purity:
Protein A/G affinity
Species Reactivity :
Human
Uniprot #:
Q9H9G7