Bcl-2 Antibody

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NSJ Bioreagents
Product Code: NSJ-V8837
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-V8837-20UG20ug£264.00
Quantity:
NSJ-V8837-100UG100ug£534.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Mouse
Antibody Isotype: Mouse IgG1, kappa
Antibody Clonality: Monoclonal
Antibody Clone: SPM117
Regulatory Status: RUO
Target Species: Human
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Aliquot the Bcl-2 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

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Western blot analysis of human MCF-7 cell lysate using Bcl-2 antibody (clone SPM117).
2 / 3
IHC staining of FFPE human tonsil tissue with Bcl-2 antibody (clone SPM117). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
3 / 3
SDS-PAGE analysis of purified, BSA-free Bcl-2 antibody (SPM117) as confirmation of integrity and purity.
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Western blot analysis of human MCF-7 cell lysate using Bcl-2 antibody (clone SPM117).
IHC staining of FFPE human tonsil tissue with Bcl-2 antibody (clone SPM117). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
SDS-PAGE analysis of purified, BSA-free Bcl-2 antibody (SPM117) as confirmation of integrity and purity.

Documents

Further Information

Application Details :
Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml
Application Note:
Optimal dilution of the Bcl-2 antibody should be determined by the researcher.
Description:
This antibody recognizes a protein of 25-26kDa, identified as the bcl-2 lpha oncoprotein. It shows no cross-reaction with Bcl-x or Bax protein. Expression of bcl-2 lpha oncoprotein inhibits the programmed cell death (apoptosis). In most follicular lymphomas, neoplastic germinal centers express high levels of bcl-2 lpha protein, whereas the normal or hyperplastic germinal centers are negative. Consequently, this antibody is valuable when distinguishing between reactive and neoplastic follicular proliferation in lymph node biopsies. It may also be used in distinguishing between those follicular lymphomas that express bcl-2 protein and the small number in which the neoplastic cells are bcl-2 negative.
Format :
Purified
Formulation :
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Immunogen:
Amino acids 41-54 (GAAPAPGIFSSQPG) from the human protein were used as the immunogen for the Bcl-2 antibody.
Limitation:
This Bcl-2 antibody is available for research use only.
Localization:
Cytoplasm, nuclear membrane
Purity:
Protein A/G affinity
Species Reactivity :
Human
Uniprot #:
P10415