HA Tag Antibody

NSJ Bioreagents
Product Code: NSJ-V8914
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-V8914-20UG20ug£264.00
Quantity:
NSJ-V8914-100UG100ug£534.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Mouse
Antibody Isotype: Mouse IgG1, kappa
Antibody Clone: HA/279
Regulatory Status: RUO
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Immunoprecipitation (IP)
  • Western Blot (WB)
Storage:
Aliquot the HA Tag antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

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SDS-PAGE analysis of purified, BSA-free HA Tag antibody (clone HA/279) as confirmation of integrity and purity.

SDS-PAGE analysis of purified, BSA-free HA Tag antibody (clone HA/279) as confirmation of integrity and purity.

Further Information

Application Details :
ELISA (order BSA-free format for coating),Immunofluorescence: 1-2ug/ml,Western blot: 1-2ug/ml,Immunoprecipitation: 1-2ug per 100-500ug of total protein (1ml of cell lysate),Flow cytometry: 1-2ug/million cells,Immunohistochemistry (FFPE): 1-2ug/ml
Application Note:
Optimal dilution of the HA Tag antibody should be determined by the researcher.
Description:
Human influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA molecule corresponding to amino acids 98-106 has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins.
Format :
Purified
Formulation :
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Immunogen:
Amino acids 98-106 (YPYDVPDYA) were used as the immunogen for the HA Tag antibody.
Limitation:
This HA Tag antibody is available for research use only.
Purity:
Protein A/G affinity
Species Reactivity :
HA tagged proteins
Uniprot #:
Not Known