Cytokeratin 5 Antibody / KRT5 (C-Terminal)

NSJ Bioreagents
Product Code: NSJ-RQ5410
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ5410-100ul100 ul£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Monoclonal
Antibody Clone: AGD-11
Regulatory Status: RUO
Target Species: Human
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store the KRT5 antibody at -20oC.

Images

1 / 2
IHC staining of FFPE human urothelial carcinoma with KRT5 antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.
2 / 2
Western blot testing of human A431 cell lysate with KRT5 antibody. Predicted molecular weight: 58-62 kDa.

IHC staining of FFPE human urothelial carcinoma with KRT5 antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.
Western blot testing of human A431 cell lysate with KRT5 antibody. Predicted molecular weight: 58-62 kDa.

Further Information

Application Details :
Western blot: 1:500-1:2000,Immunohistochemistry (FFPE): 1:50-1:200
Application Note:
Optimal dilution of the KRT5 antibody should be determined by the researcher.
Description:
The protein encoded by the KRT5 gene is a member of the keratin gene family. The type II cytokeratins consist of basic or neutral proteins which are arranged in pairs of heterotypic keratin chains coexpressed during differentiation of simple and stratified epithelial tissues. This type II cytokeratin is specifically expressed in the basal layer of the epidermis with family member KRT14. Mutations in these genes have been associated with a complex of diseases termed epidermolysis bullosa simplex. The type II cytokeratins are clustered in a region of chromosome 12q12-q13. [RefSeq]
Format :
Purified
Formulation :
Antibody in PBS with 0.02% sodium azide and 50% glycerol
Immunogen:
A synthetic peptide specific to the C-terminal region of human Cytokeratin 5 / KRT5 was used as the immunogen for the KRT5 antibody.
Limitation:
This KRT5 antibody is available for research use only.
Localization:
Cytoplasmic
Purity:
Affinity purified
Species Reactivity :
Human
Uniprot #:
P13647