Phospho-BRAF Antibody (pT401)

Western blot: 1:500-1:2000

Optimal dilution of the phospho-B Raf antibody (pT401) should be determined by the researcher.

The BRAF gene encodes a protein belonging to the RAF family of serine/threonine protein kinases. This protein plays a role in regulating the MAP kinase/ERK signaling pathway, which affects cell division, differentiation, and secretion. Mutations in this gene, most commonly the V600E mutation, are the most frequently identified cancer-causing mutations in melanoma, and have been identified in various other cancers as well, including non-Hodgkin lymphoma, colorectal cancer, thyroid carcinoma, non-small cell lung carcinoma, hairy cell leukemia and adenocarcinoma of lung. Mutations in this gene are also associated with cardiofaciocutaneous, Noonan, and Costello syndromes, which exhibit overlapping phenotypes. [RefSeq]

Purified

Antibody in PBS with 0.02% sodium azide and 50% glycerol

A synthetic peptide specific to human B Raf (surrounding pT401) was used as the immunogen for the phospho-B Raf antibody.

This phospho-B Raf antibody (pT401) is available for research use only.

Affinity purified

Human, Mouse, Rat

P15056