Phospho-CREB1 Antibody (pS133)

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NSJ Bioreagents
Product Code: NSJ-RQ5477
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ5477-100ul100 ul£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Monoclonal
Antibody Clone: IG-3
Regulatory Status: RUO
Target Species: Human
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store the phospho-CREB1 antibody (pS133) at -20oC.

Images

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Western blot testing of lysate from human HeLa cells treated with Calyculin A, with phospho-CREB1 antibody (pS133). Expected molecular weight: 37-43 kDa.
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IHC staining of FFPE human gastric carcinoma with phospho-CREB1 antibody (pS133) antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.
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Western blot testing of lysate from human HeLa cells treated with Calyculin A, with phospho-CREB1 antibody (pS133). Expected molecular weight: 37-43 kDa.
IHC staining of FFPE human gastric carcinoma with phospho-CREB1 antibody (pS133) antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.

Documents

Further Information

Application Details :
Western blot: 1:500-1:2000,Immunohistochemistry (FFPE): 1:50-1:200
Application Note:
Optimal dilution of the phospho-CREB1 antibody (pS133) should be determined by the researcher.
Description:
The CREB1 gene encodes a transcription factor that is a member of the leucine zipper family of DNA binding proteins. This protein binds as a homodimer to the cAMP-responsive element, an octameric palindrome. The protein is phosphorylated by several protein kinases, and induces transcription of genes in response to hormonal stimulation of the cAMP pathway. Alternate splicing of this gene results in several transcript variants encoding different isoforms. [RefSeq]
Format :
Purified
Formulation :
Antibody in PBS with 0.02% sodium azide and 50% glycerol
Immunogen:
A synthetic peptide specific to human CREB (surrounding pS133) was used as the immunogen for the CREB1 antibody.
Limitation:
This phospho-CREB1 antibody (pS133) is available for research use only.
Localization:
Nuclear
Purity:
Affinity purified
Species Reactivity :
Human
Uniprot #:
P16220