BRG1 Antibody / SMARCA4

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NSJ Bioreagents
Product Code: NSJ-RQ5493
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ5493-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Mouse
Antibody Isotype: Mouse IgG1
Antibody Clonality: Monoclonal
Antibody Clone: 3F4
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the BRG1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Images

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IHC staining of FFPE human rectal cancer with BRG1 antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.
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IHC staining of FFPE human rectal cancer with BRG1 antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.
3 / 6
IHC staining of FFPE human lung cancer with BRG1 antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.
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IHC staining of FFPE rat intestine with BRG1 antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.
5 / 6
Western blot testing of human 1) Raji, 2) K562, 3) HL-60, 4) Caco-2, 5) HeLa, 6) HepG2, 7) rat brain, 8) mouse brain and 9) mouse lung with BRG1 antibody. Predicted molecular weight: 185-190 kDa, observed at 200-220 kDa.
6 / 6
Flow cytometry testing of human U-2 OS cells with BRG1 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= BRG1 antibody.
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IHC staining of FFPE human rectal cancer with BRG1 antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.
IHC staining of FFPE human rectal cancer with BRG1 antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.
IHC staining of FFPE human lung cancer with BRG1 antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.
IHC staining of FFPE rat intestine with BRG1 antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.
Western blot testing of human 1) Raji, 2) K562, 3) HL-60, 4) Caco-2, 5) HeLa, 6) HepG2, 7) rat brain, 8) mouse brain and 9) mouse lung with BRG1 antibody. Predicted molecular weight: 185-190 kDa, observed at 200-220 kDa.
Flow cytometry testing of human U-2 OS cells with BRG1 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= BRG1 antibody.

Documents

Further Information

Application Details :
Western blot: 0.5-1ug/ml, Immunohistochemistry (FFPE): 1-2ug/ml, Flow cytometry: 1-3ug/million cells
Application Note:
Optimal dilution of the BRG1 antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide
Description:
Transcription activator BRG1 also known as ATP-dependent helicase SMARCA4 is a protein that in humans is encoded by the SMARCA4 gene. The protein encoded by this gene is a member of the SWI/SNF family of proteins and is similar to the brahma protein of Drosophila. Members of this family have helicase and ATPase activities and are thought to regulate transcription of certain genes by altering the chromatin structure around those genes. The encoded protein is part of the large ATP-dependent chromatin remodeling complex SNF/SWI, which is required for transcriptional activation of genes normally repressed by chromatin. In addition, this protein can bind BRCA1, as well as regulate the expression of the tumorigenic protein CD44. Mutations in this gene cause rhabdoid tumor predisposition syndrome type 2. Multiple transcript variants encoding different isoforms have been found for this gene.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
A human recombinant protein (amino acids Q555-E763) was used as the immunogen for the BRG1 antibody.
Limitation:
This BRG1 antibody is available for research use only.
Localization:
Nuclear
Purity:
Affinity purified
Species Reactivity :
Human, Mouse, Rat
Uniprot #:
P51532