CDK1 Antibody

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NSJ Bioreagents
Product Code: NSJ-RQ5499
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ5499-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Mouse
Antibody Isotype: Mouse IgG2b
Antibody Clonality: Monoclonal
Antibody Clone: 2G11
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the CDK1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Images

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Flow cytometry testing of human U-2 OS cells with CDK1 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= CDK1 antibody.
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Flow cytometry testing of human PC-3 cells with CDK1 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= CDK1 antibody.
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Western blot testing of human 1) HEK293, 2) A549, 3) HepG2, 4) ThP-1, 5) PANC-1, 6) SW620, 7) rat RH30 and 8) mouse NIH3T3 lysate with CDK1 antibody. Predicted molecular weight ~33 kDa.
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IHC staining of FFPE human colon cancer with CDK1 antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.
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IHC staining of FFPE human tonsil with CDK1 antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.
6 / 8
IHC staining of FFPE human lung cancer with CDK1 antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.
7 / 8
IHC staining of FFPE mouse testis with CDK1 antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.
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IHC staining of FFPE rat testis with CDK1 antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.
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Flow cytometry testing of human U-2 OS cells with CDK1 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= CDK1 antibody.
Flow cytometry testing of human PC-3 cells with CDK1 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= CDK1 antibody.
Western blot testing of human 1) HEK293, 2) A549, 3) HepG2, 4) ThP-1, 5) PANC-1, 6) SW620, 7) rat RH30 and 8) mouse NIH3T3 lysate with CDK1 antibody. Predicted molecular weight ~33 kDa.
IHC staining of FFPE human colon cancer with CDK1 antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.
IHC staining of FFPE human tonsil with CDK1 antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.
IHC staining of FFPE human lung cancer with CDK1 antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.
IHC staining of FFPE mouse testis with CDK1 antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.
IHC staining of FFPE rat testis with CDK1 antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.

Documents

Further Information

Application Details :
Western blot: 0.5-1ug/ml, Immunohistochemistry (FFPE): 1-2ug/ml, Flow cytometry: 1-3ug/million cells
Application Note:
Optimal dilution of the CDK1 antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide
Description:
CDC2, Cell Division Cycle 2, is also known as CDK1 (Cyclin-dependent Kinase 1). CDC2 is a catalytic subunit of a protein kinase complex, called the M-phase promoting factor that induces entry into mitosis and is universal among eukaryotes. In HeLa cells CDC2 is the most abundant phosphotyrosine-containing protein and its phosphotyrosine content is subject to cell cycle regulation. CDC2 gene is located on chromosome 10.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
A human recombinant protein (amino acids L66-M297) was used as the immunogen for the CDK1 antibody.
Limitation:
This CDK1 antibody is available for research use only.
Purity:
Affinity purified
Species Reactivity :
Human, Mouse, Rat
Uniprot #:
P06493