PPCS Antibody / Phosphopantothenoylcysteine synthetase

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NSJ Bioreagents
Product Code: NSJ-RQ5506
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ5506-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Mouse
Antibody Isotype: Mouse IgG2a
Antibody Clonality: Monoclonal
Antibody Clone: 7G13
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the PPCS antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Images

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IHC staining of FFPE human tonsil with PPCS antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.
2 / 6
IHC staining of FFPE mouse heart with PPCS antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.
3 / 6
IHC staining of FFPE rat heart with PPCS antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.
4 / 6
Western blot testing of human 1) A375 and 2) COLO-320 cell lysate with PPCS antibody. Predicted molecular weight ~34 kDa.
5 / 6
Flow cytometry testing of human PC-3 cells with PPCS antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= PPCS antibody.
6 / 6
Flow cytometry testing of human SiHa cells with PPCS antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= PPCS antibody.
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IHC staining of FFPE human tonsil with PPCS antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.
IHC staining of FFPE mouse heart with PPCS antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.
IHC staining of FFPE rat heart with PPCS antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.
Western blot testing of human 1) A375 and 2) COLO-320 cell lysate with PPCS antibody. Predicted molecular weight ~34 kDa.
Flow cytometry testing of human PC-3 cells with PPCS antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= PPCS antibody.
Flow cytometry testing of human SiHa cells with PPCS antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= PPCS antibody.

Documents

Further Information

Application Details :
Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml,Flow cytometry: 1-3ug/million cells
Application Note:
Optimal dilution of the PPCS antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide
Description:
Phosphopantothenoylcysteine synthetase in humans is encoded by the PPCS gene. Biosynthesis of coenzyme A (CoA) from pantothenic acid (vitamin B5) is an essential universal pathway in prokaryotes and eukaryotes. PPCS, one of the last enzymes in this pathway, converts phosphopantothenate to phosphopantothenoylcysteine. By genomic sequence analysis, this PPCS gene ia mapped to chromosome 1.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Amino acids VEIEEKIVDNLQSRHTAFIGDRN were used as the immunogen for the PPCS antibody.
Limitation:
This PPCS antibody is available for research use only.
Purity:
Affinity purified
Species Reactivity :
Human, Mouse, Rat
Uniprot #:
Q9HAB8