HSP105 Antibody / HSPH1

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NSJ Bioreagents
Product Code: NSJ-RQ5517
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ5517-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Mouse
Antibody Isotype: Mouse IgG1
Antibody Clonality: Monoclonal
Antibody Clone: 3D10
Regulatory Status: RUO
Target Species: Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the Hsp105 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Images

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IHC staining of FFPE human intestinal cancer with Hsp105 Antibody antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.
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IHC staining of FFPE human breast cancer with Hsp105 Antibody antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.
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IHC staining of FFPE human lung cancer with Hsp105 Antibody antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.
4 / 8
Immunofluorescent staining of FFPE human A431 cells with Hsp105 antibody (green) and DAPI (blue). HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.
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Western blot testing of human 1) Caco-2, 2) K562, 3) A549, 4) HepG2, 5) PANC-1 and 6) SGC-7901 cell lysate with Hsp105 antibody. Expected molecular weight: 105-110 kDa.
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Flow cytometry testing of human HepG2 cells with Hsp105 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Hsp105 antibody.
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Flow cytometry testing of human A431 cells with Hsp105 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Hsp105 antibody.
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Immunofluorescent staining of FFPE human MCF7 cells with Hsp105 antibody (green) and DAPI (blue). HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.
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IHC staining of FFPE human intestinal cancer with Hsp105 Antibody antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.
IHC staining of FFPE human breast cancer with Hsp105 Antibody antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.
IHC staining of FFPE human lung cancer with Hsp105 Antibody antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.
Immunofluorescent staining of FFPE human A431 cells with Hsp105 antibody (green) and DAPI (blue). HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.
Western blot testing of human 1) Caco-2, 2) K562, 3) A549, 4) HepG2, 5) PANC-1 and 6) SGC-7901 cell lysate with Hsp105 antibody. Expected molecular weight: 105-110 kDa.
Flow cytometry testing of human HepG2 cells with Hsp105 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Hsp105 antibody.
Flow cytometry testing of human A431 cells with Hsp105 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Hsp105 antibody.
Immunofluorescent staining of FFPE human MCF7 cells with Hsp105 antibody (green) and DAPI (blue). HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.

Documents

Further Information

Application Details :
Western blot: 0.5-1ug/ml, Immunohistochemistry (FFPE): 1-2ug/ml,Immunofluorescence: 2-4ug/ml, Flow cytometry: 1-3ug/million cells
Application Note:
Optimal dilution of the Hsp105 antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide
Description:
HSP105 (HEAT-SHOCK 105/110-KD PROTEIN 1), also called HSPH1 or HSP110, is a protein that in humans is encoded by the HSPH1 gene. Immunohistochemical analysis localizes HSP105 mainly in the cytoplasm. Database analysis indicates that both HSP105 isoforms are highly conserved during evolution. By analysis of radiation hybrids and human/rodent hybrid cell lines, the HSPH1 gene is mapped to chromosome 13. Both HSP105-alpha and HSP105-beta are upregulated in HeLa cells exposed to heat shock. HSP105-alpha, but not HSP105-beta, is also upregulate in response to other cell stresses. Following heat shock, HSP105 relocalizes from a cytoplasmic to perinuclear position. Besides, HSP110 may thus constitute a major determinant for both prognosis and treatment response in colorectal cancer.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
A human recombinant protein (amino acids Y653-D858) was used as the immunogen for the Hsp105 antibody.
Limitation:
This Hsp105 antibody is available for research use only.
Purity:
Affinity purified
Species Reactivity :
Human
Uniprot #:
Q92598