EIF4A1/2/3 Antibody

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NSJ Bioreagents
Product Code: NSJ-RQ5520
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ5520-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the EIF4A1/2/3 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Images

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Western blot testing of human 1) placenta 2) HEK293, 3) A549, 4) HepG2, 5) U-87 MG, 6) PC-3 and 7) K562 lysate with EIF4A1/2/3 antibody. Predicted molecular weight ~46 kDa.
2 / 9
Western blot testing of 1) rat thymus, 2) mouse thymus, 3) mouse lung and 7) mouse SP20 lysate with EIF4A1/2/3 antibody. Predicted molecular weight ~46 kDa.
3 / 9
Immunofluorescent staining of FFPE human U-2 OS cells with EIF4A1/2/3 antibody (green) and DAPI (blue). HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.
4 / 9
IHC staining of FFPE human colon cancer with EIF4A1/2/3 antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.
5 / 9
IHC staining of FFPE human lung cancer with EIF4A1/2/3 antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.
6 / 9
IHC staining of FFPE mouse intestine with EIF4A1/2/3 antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.
7 / 9
IHC staining of FFPE rat intestine with EIF4A1/2/3 antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.
8 / 9
Flow cytometry testing of human U-87 MG cells with EIF4A1/2/3 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= EIF4A1/2/3 antibody.
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Flow cytometry testing of human HepG2 cells with EIF4A1/2/3 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= EIF4A1/2/3 antibody.
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Western blot testing of human 1) placenta 2) HEK293, 3) A549, 4) HepG2, 5) U-87 MG, 6) PC-3 and 7) K562 lysate with EIF4A1/2/3 antibody. Predicted molecular weight ~46 kDa.
Western blot testing of 1) rat thymus, 2) mouse thymus, 3) mouse lung and 7) mouse SP20 lysate with EIF4A1/2/3 antibody. Predicted molecular weight ~46 kDa.
Immunofluorescent staining of FFPE human U-2 OS cells with EIF4A1/2/3 antibody (green) and DAPI (blue). HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.
IHC staining of FFPE human colon cancer with EIF4A1/2/3 antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.
IHC staining of FFPE human lung cancer with EIF4A1/2/3 antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.
IHC staining of FFPE mouse intestine with EIF4A1/2/3 antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.
IHC staining of FFPE rat intestine with EIF4A1/2/3 antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.
Flow cytometry testing of human U-87 MG cells with EIF4A1/2/3 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= EIF4A1/2/3 antibody.
Flow cytometry testing of human HepG2 cells with EIF4A1/2/3 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= EIF4A1/2/3 antibody.

Documents

Further Information

Application Details :
Western blot: 0.25-0.5ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml,Immunofluorescence: 2-4ug/ml,Flow cytometry: 1-3ug/million cells,Direct ELISA: 0.1-0.5ug/ml
Application Note:
Optimal dilution of the EIF4A1/2/3 antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide
Description:
Eukaryotic initiation factor 4A-I is a protein that in humans is encoded by the EIF4A1 gene. It is mapped to 17p13.1. EIF4A1 has been shown to interact with EIF4E and eukaryotic translation initiation factor 4 gamma.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
A human recombinant protein (amino acids D32-I406 ) was used as the immunogen for the EIF4A1/2/3 antibody.
Limitation:
This EIF4A1/2/3 antibody is available for research use only.
Purity:
Affinity purified
Species Reactivity :
Human, Mouse, Rat
Uniprot #:
P60842