PKC delta Antibody

Contact us
NSJ Bioreagents
Product Code: NSJ-RQ5529
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ5529-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the PRKCD antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Images

1 / 10
Western blot testing of human 1) placenta, 2) ThP-1, 3) Caco-2 and 4) HEK293 lysate with PRKCD antibody. Predicted molecular weight ~77 kDa.
2 / 10
Western blot testing of 1) rat brain, 2) mouse brain, 3) mouse thymus and 4) mouse lung lysate with PRKCD antibody. Predicted molecular weight ~77 kDa.
3 / 10
Immunofluorescent staining of FFPE human U-2 OS cells with PRKCD antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.
4 / 10
Flow cytometry testing of human ThP-1 cells with PRKCD antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= PRKCD antibody.
5 / 10
Flow cytometry testing of human Caco-2 cells with PRKCD antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= PRKCD antibody.
6 / 10
IHC staining of FFPE human breast cancer with PRKCD antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.
7 / 10
IHC staining of FFPE human colon cancer with PRKCD antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.
8 / 10
IHC staining of FFPE mouse intestine with PRKCD antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.
9 / 10
IHC staining of FFPE rat intestine with PRKCD antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.
10 / 10
Immunofluorescent staining of FFPE human A549 cells with PRKCD antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.
❮❯

Western blot testing of human 1) placenta, 2) ThP-1, 3) Caco-2 and 4) HEK293 lysate with PRKCD antibody. Predicted molecular weight ~77 kDa.
Western blot testing of 1) rat brain, 2) mouse brain, 3) mouse thymus and 4) mouse lung lysate with PRKCD antibody. Predicted molecular weight ~77 kDa.
Immunofluorescent staining of FFPE human U-2 OS cells with PRKCD antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.
Flow cytometry testing of human ThP-1 cells with PRKCD antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= PRKCD antibody.
Flow cytometry testing of human Caco-2 cells with PRKCD antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= PRKCD antibody.
IHC staining of FFPE human breast cancer with PRKCD antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.
IHC staining of FFPE human colon cancer with PRKCD antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.
IHC staining of FFPE mouse intestine with PRKCD antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.
IHC staining of FFPE rat intestine with PRKCD antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.
Immunofluorescent staining of FFPE human A549 cells with PRKCD antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.

Documents

Further Information

Application Details :
Western blot: 0.25-0.5ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml,Immunofluorescence: 2-4ug/ml,Flow cytometry: 1-3ug/million cells,Direct ELISA: 0.1-0.5ug/ml
Application Note:
Optimal dilution of the PRKCD antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide
Description:
Protein kinase C delta type, also called PKCD, is an enzyme that in humans is encoded by the PRKCD gene. The PRKCD gene encodes a member of the protein kinase C family, members of which are critical for regulation of cell survival, proliferation, and apoptosis. This gene is mapped to 3p21.1. Studies both in human and mice demonstrate that this kinase is involved in B cell signaling and in the regulation of growth, apoptosis, and differentiation of a variety of cell types. PRKCD is also regulated by phosphorylation on various tyrosine residues including Y311 (by SRC). It has been showed that PRKCD phosphorylates NLRC4 and that this phosphorylation is critical for inflammasome assembly. It is also a part of a noncanonical WNT signaling cascade.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
A human recombinant protein (amino acids F4-D676) was used as the immunogen for the PRKCD antibody.
Limitation:
This PRKCD antibody is available for research use only.
Localization:
Cytoplasmic, nuclear, plasma membrane
Purity:
Affinity purified
Species Reactivity :
Human, Mouse, Rat
Uniprot #:
Q05655