FN1 Antibody / Fibronectin

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NSJ Bioreagents
Product Code: NSJ-RQ5531
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ5531-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Western Blot (WB)
Storage:
After reconstitution, the Fibronectin antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Images

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Western blot testing of human U-87 MG cell lysate with Fibronectin antibody. Predicted molecular weight ~220 kDa (monomer) but may be observed at a higher molecular weight due to glycosylation.
2 / 3
Flow cytometry testing of human HeLa cells with Fibronectin antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Fibronectin antibody.
3 / 3
Flow cytometry testing of human Caco-2 cells with Fibronectin antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Fibronectin antibody.
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Western blot testing of human U-87 MG cell lysate with Fibronectin antibody. Predicted molecular weight ~220 kDa (monomer) but may be observed at a higher molecular weight due to glycosylation.
Flow cytometry testing of human HeLa cells with Fibronectin antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Fibronectin antibody.
Flow cytometry testing of human Caco-2 cells with Fibronectin antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Fibronectin antibody.

Documents

Further Information

Application Details :
Western blot: 0.25-0.5ug/ml,Flow cytometry: 1-3ug/million cells,Direct ELISA: 0.1-0.5ug/ml
Application Note:
Optimal dilution of the Fibronectin antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide
Description:
Fibronectin is a high-molecular weight glycoprotein of the extracellular matrix that binds to membrane-spanning receptor proteins called integrins. It is mapped to 2q35. This gene encodes fibronectin, a glycoprotein present in a soluble dimeric form in plasma, and in a dimeric or multimeric form at the cell surface and in extracellular matrix. The encoded preproprotein is proteolytically processed to generate the mature protein. Fibronectin is involved in cell adhesion and migration processes including embryogenesis, wound healing, blood coagulation, host defense, and metastasis. The gene has three regions subject to alternative splicing, with the potential to produce 20 different transcript variants, at least one of which encodes an isoform that undergoes proteolytic processing. The full-length nature of some variants has not been determined.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
A human recombinant protein (amino acids T1877-E2386) was used as the immunogen for the Fibronectin antibody.
Limitation:
This Fibronectin antibody is available for research use only.
Purity:
Affinity purified
Species Reactivity :
Human
Uniprot #:
P02751