MTTP Antibody / MTP

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NSJ Bioreagents
Product Code: NSJ-RQ5538
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ5538-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Western Blot (WB)
Storage:
After reconstitution, the MTTP antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Images

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Western blot testing of human HepG2 cell lysate with MTTP antibody. Predicted molecular weight ~99 kDa.
2 / 3
Immunofluorescent staining of FFPE human U-2 OS cells with MTTP antibody antibody (green) and DAPI (blue). HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.
3 / 3
Flow cytometry testing of human HepG2 cells with MTTP antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= MTTP antibody.
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Western blot testing of human HepG2 cell lysate with MTTP antibody. Predicted molecular weight ~99 kDa.
Immunofluorescent staining of FFPE human U-2 OS cells with MTTP antibody antibody (green) and DAPI (blue). HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.
Flow cytometry testing of human HepG2 cells with MTTP antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= MTTP antibody.

Documents

Further Information

Application Details :
Western blot: 0.25-0.5ug/ml,Immunofluorescence: 2-4ug/ml,Flow cytometry: 1-3ug/million cells,Direct ELISA: 0.1-0.5ug/ml
Application Note:
Optimal dilution of the MTTP antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide
Description:
Microsomal triglyceride transfer protein large subunit is a protein that in humans is encoded by the MTTP gene. It is mapped to 4q23. MTP encodes the large subunit of the heterodimeric microsomal triglyceride transfer protein. Protein disulfide isomerase (PDI) completes the heterodimeric microsomal triglyceride transfer protein, which has been shown to play a central role in lipoprotein assembly. Mutations in MTP can cause abetalipoproteinemia.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
A human recombinant protein (amino acids N29-R702) was used as the immunogen for the MTTP antibody.
Limitation:
This MTTP antibody is available for research use only.
Purity:
Affinity purified
Species Reactivity :
Human
Uniprot #:
P55157