SYK Antibody / Spleen tyrosine kinase

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NSJ Bioreagents
Product Code: NSJ-RQ5553
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ5553-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Western Blot (WB)
Storage:
After reconstitution, the SYK antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Images

1 / 5
Western blot testing of human 1) placenta and 2) ThP-1 lysate with SYK antibody. Predicted molecular weight ~72 kDa.
2 / 5
Western blot testing of mouse 1) thymus, 2) lung and 3) SP20 lysate with SYK antibody. Predicted molecular weight ~72 kDa.
3 / 5
Western blot testing of rat thymus lysate with SYK antibody. Predicted molecular weight ~72 kDa.
4 / 5
Flow cytometry testing of human ThP-1 cells with SYK antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= SYK antibody.
5 / 5
Flow cytometry testing of human A431 cells with SYK antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= SYK antibody.
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Western blot testing of human 1) placenta and 2) ThP-1 lysate with SYK antibody. Predicted molecular weight ~72 kDa.
Western blot testing of mouse 1) thymus, 2) lung and 3) SP20 lysate with SYK antibody. Predicted molecular weight ~72 kDa.
Western blot testing of rat thymus lysate with SYK antibody. Predicted molecular weight ~72 kDa.
Flow cytometry testing of human ThP-1 cells with SYK antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= SYK antibody.
Flow cytometry testing of human A431 cells with SYK antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= SYK antibody.

Documents

Further Information

Application Details :
Western blot: 0.25-0.5ug/ml,Flow cytometry: 1-3ug/million cells,Direct ELISA: 0.1-0.5ug/ml
Application Note:
Optimal dilution of the SYK antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide
Description:
Spleen tyrosine kinase, also known as Syk, is an enzyme which in humans is encoded by the SYK gene. It is mapped to 9q22.2. This gene encodes a member of the family of non-receptor type Tyr protein kinases. This protein is widely expressed in hematopoietic cells and is involved in coupling activated immunoreceptors to downstream signaling events that mediate diverse cellular responses, including proliferation, differentiation, and phagocytosis. It is thought to be a modulator of epithelial cell growth and a potential tumour suppressor in human breast carcinomas. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
A human recombinant protein (amino acids A10-N635) was used as the immunogen for the SYK antibody.
Limitation:
This SYK antibody is available for research use only.
Purity:
Affinity purified
Species Reactivity :
Human, Mouse, Rat
Uniprot #:
P43405