BLyS Antibody / BAFF

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NSJ Bioreagents
Product Code: NSJ-RQ5555
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ5555-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Western Blot (WB)
Storage:
After reconstitution, the BLyS antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Images

1 / 4
Western blot testing of human 1) U-937, 2) Caco-2, 3) PANC-1, 4) CCRF-CEM, 5) MDA-MB-231 lysate with BLyS antibody. Predicted molecular weight ~34 kDa.
2 / 4
Western blot testing of 1) rat testis and 2) mouse testis lysate with BLyS antibody. Predicted molecular weight ~34 kDa.
3 / 4
Flow cytometry testing of human HL60 cells with BLyS antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= BLyS antibody.
4 / 4
Flow cytometry testing of human U-87 MG cells with BLyS antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= BLyS antibody.
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Western blot testing of human 1) U-937, 2) Caco-2, 3) PANC-1, 4) CCRF-CEM, 5) MDA-MB-231 lysate with BLyS antibody. Predicted molecular weight ~34 kDa.
Western blot testing of 1) rat testis and 2) mouse testis lysate with BLyS antibody. Predicted molecular weight ~34 kDa.
Flow cytometry testing of human HL60 cells with BLyS antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= BLyS antibody.
Flow cytometry testing of human U-87 MG cells with BLyS antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= BLyS antibody.

Documents

Further Information

Application Details :
Western blot: 0.25-0.5ug/ml,Flow cytometry: 1-3ug/million cells,Direct ELISA: 0.1-0.5ug/ml
Application Note:
Optimal dilution of the BLyS antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide
Description:
BLyS / BAFF was regularly detected by enzyme-linked immunosorbent assay in brain tissue lysates and in normal spinal fluid, and in astrocytes by double fluorescence microscopy. BAFF was localized in astrocytes close to BAFF-R-expressing immune cells. BAFF receptors were strongly expressed in situ in primary central nervous system (CNS) lymphomas. The TNF superfamily member B cell-activating factor (BAFF) plays an important role in humoral immunity and in autoimmune diseases, including RA. Local BAFF gene targeting inhibited proinflammatory cytokine expression, suppressed generation of plasma cells and Th17 cells, and markedly ameliorated joint pathology. The B cell activating factor BAFF (BlyS/TALL-1/zTNF4) is a tumor necrosis factor (TNF)-related ligand that promotes B cell survival and binds to three receptors (BCMA, TACI, and the recently described BAFF-R).
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
A human recombinant protein (amino acids A134-T277) was used as the immunogen for the BLyS antibody.
Limitation:
This BLyS antibody is available for research use only.
Purity:
Affinity purified
Species Reactivity :
Human, Mouse, Rat
Uniprot #:
Q9Y275