TNNT3 Antibody

Western blot: 0.25-0.5ug/ml,Direct ELISA: 0.1-0.5ug/ml

Optimal dilution of the TNNT3 antibody should be determined by the researcher.

Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide

Fast skeletal muscle troponin T (fTnT) is a protein that in humans is encoded by the TNNT3 gene. It is mapped to 11p15.5. The binding of Ca(2+) to the trimeric troponin complex initiates the process of muscle contraction. Increased Ca(2+) concentrations produce a conformational change in the troponin complex that is transmitted to tropomyosin dimers situated along actin filaments. The altered conformation permits increased interaction between a myosin head and an actin filament which, ultimately, produces a muscle contraction. The troponin complex has protein subunits C, I, and T. Subunit C binds Ca(2+) and subunit I binds to actin and inhibits actin-myosin interaction. Subunit T binds the troponin complex to the tropomyosin complex and is also required for Ca(2+)-mediated activation of actomyosin ATPase activity. There are 3 different troponin T genes that encode tissue-specific isoforms of subunit T for fast skeletal-, slow skeletal-, and cardiac-muscle. This gene encodes fast skeletal troponin T protein; also known as troponin T type 3. Alternative splicing results in multiple transcript variants encoding additional distinct troponin T type 3 isoforms. A developmentally regulated switch between fetal/neonatal and adult troponin T type 3 isoforms occurs. Additional splice variants have been described but their biological validity has not been established. Mutations in this gene may cause distal arthrogryposis multiplex congenita type 2B (DA2B).

Antigen affinity purified

0.5mg/ml if reconstituted with 0.2ml sterile DI water

A human recombinant protein (amino acids A45-K251) was used as the immunogen for the TNNT3 antibody.

This TNNT3 antibody is available for research use only.

Affinity purified

Human, Mouse, Rat

P45378