DCK Antibody / Deoxycytidine kinase

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NSJ Bioreagents
Product Code: NSJ-RQ5558
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ5558-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Mouse
Antibody Isotype: Mouse IgG2b
Antibody Clonality: Monoclonal
Antibody Clone: 3G10
Regulatory Status: RUO
Target Species: Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the DCK antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Images

1 / 4
IHC staining of FFPE human tonsil with DCK antibody. HIER: boil tissue sections in pH8 EDTA buffer, for 20 min and allow to cool before testing.
2 / 4
Immunofluorescent staining of human U-2 OS cells with DCK antibody.
3 / 4
Western blot testing of human 1) HEK293, 2) HeLa, 3) HepG2, 4) Jurkat and 5) Raji cell lysate with DCK antibody. Predicted molecular weight ~30 kDa.
4 / 4
Flow cytometry testing of human 293T cells with DCK antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= DCK antibody.
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IHC staining of FFPE human tonsil with DCK antibody. HIER: boil tissue sections in pH8 EDTA buffer, for 20 min and allow to cool before testing.
Immunofluorescent staining of human U-2 OS cells with DCK antibody.
Western blot testing of human 1) HEK293, 2) HeLa, 3) HepG2, 4) Jurkat and 5) Raji cell lysate with DCK antibody. Predicted molecular weight ~30 kDa.
Flow cytometry testing of human 293T cells with DCK antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= DCK antibody.

Documents

Further Information

Application Details :
Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml,Flow cytometry: 1-3ug/million cells
Application Note:
Optimal dilution of the DCK antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide
Description:
Deoxycytidine kinase (dCK) is an enzyme which is encoded by the DCK gene in humans. Deoxycytidine kinase (DCK) is required for the phosphorylation of several deoxyribonucleosides and their nucleoside analogs. Deficiency of DCK is associated with resistance to antiviral and anticancer chemotherapeutic agents. Conversely, increased deoxycytidine kinase activity is associated with increased activation of these compounds to cytotoxic nucleoside triphosphate derivatives. DCK is clinically important because of its relationship to drug resistance and sensitivity.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
A human recombinant protein (amino acids E17-L260) was used as the immunogen for the DCK antibody.
Limitation:
This DCK antibody is available for research use only.
Localization:
Nuclear, cytoplasmic
Purity:
Affinity purified
Species Reactivity :
Human
Uniprot #:
P27707