Exonuclease 1 Antibody / EXO1

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NSJ Bioreagents
Product Code: NSJ-RQ5576
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ5576-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Western Blot (WB)
Storage:
After reconstitution, the Exonuclease 1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Images

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Western blot testing of human 1) placenta, 2) K562, 3) MDA-MB-453, 4) PC-3, 5) PANC-1, 6) HEK293 and 7) U-2 OS lysate with Exonuclease 1 antibody. Expected molecular weight: 94-115 kDa.
2 / 2
Flow cytometry testing of human 293T cells with Exonuclease 1 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Exonuclease 1 antibody.
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Western blot testing of human 1) placenta, 2) K562, 3) MDA-MB-453, 4) PC-3, 5) PANC-1, 6) HEK293 and 7) U-2 OS lysate with Exonuclease 1 antibody. Expected molecular weight: 94-115 kDa.
Flow cytometry testing of human 293T cells with Exonuclease 1 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Exonuclease 1 antibody.

Documents

Further Information

Application Details :
Western blot: 0.25-0.5ug/ml,Flow cytometry: 1-3ug/million cells,Direct ELISA: 0.1-0.5ug/ml
Application Note:
Optimal dilution of the Exonuclease 1 antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide
Description:
Exonuclease 1is an enzyme that in humans is encoded by the EXO1 gene. It is mapped to 1q43. This gene encodes a protein with 5' to 3' exonuclease activity as well as an RNase H activity. It is similar to the Saccharomyces cerevisiae protein Exo1 which interacts with Msh2 and which is involved in mismatch repair and recombination. Alternative splicing of this gene results in three transcript variants encoding two different isoforms.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
A human recombinant protein (amino acids M1-K294) was used as the immunogen for the Exonuclease 1 antibody.
Limitation:
This Exonuclease 1 antibody is available for research use only.
Purity:
Affinity purified
Species Reactivity :
Human
Uniprot #:
Q9UQ84