Beta Glucuronidase Antibody / Gusb

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NSJ Bioreagents
Product Code: NSJ-RQ5581
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ5581-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunocytochemistry (ICC)
  • Immunofluorescence (IF)
  • Western Blot (WB)
Storage:
After reconstitution, the Beta Glucuronidase antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Images

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Immunofluorescent staining of FFEP human A431 cells with Beta Glucuronidase antibody (red) and DAPI nuclear stain (blue). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
2 / 4
Flow cytometry testing of human Caco-2 cells with Beta Glucuronidase antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Beta Glucuronidase antibody.
3 / 4
Flow cytometry testing of human A431 cells with Beta Glucuronidase antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Beta Glucuronidase antibody.
4 / 4
Western blot testing of human 1) K562, 2) Caco-2, 3) U-87 MG and 4) HepG2 cell lysate with Beta Glucuronidase antibody. Expected molecular weight: 68-75 kDa, also seen as dimers and tetramers.
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Immunofluorescent staining of FFEP human A431 cells with Beta Glucuronidase antibody (red) and DAPI nuclear stain (blue). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
Flow cytometry testing of human Caco-2 cells with Beta Glucuronidase antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Beta Glucuronidase antibody.
Flow cytometry testing of human A431 cells with Beta Glucuronidase antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Beta Glucuronidase antibody.
Western blot testing of human 1) K562, 2) Caco-2, 3) U-87 MG and 4) HepG2 cell lysate with Beta Glucuronidase antibody. Expected molecular weight: 68-75 kDa, also seen as dimers and tetramers.

Documents

Further Information

Application Details :
Western blot: 0.25-0.5ug/ml,Flow cytometry: 1-3ug/million cells,Immunofluorescence: 2-4ug/ml,Direct ELISA: 0.1-0.5ug/ml
Application Note:
Optimal dilution of the Beta Glucuronidase antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide
Description:
Beta-glucuronidases are members of the glycosidase family of enzymes that catalyze breakdown of complex carbohydrates. This gene encodes a hydrolase that degrades glycosaminoglycans, including heparan sulfate, dermatan sulfate, and chondroitin-4,6-sulfate. The enzyme forms a homotetramer that is localized to the lysosome. Mutations in this gene result in mucopolysaccharidosis type VII. Alternative splicing results in multiple transcript variants. There are many pseudogenes of this locus in the human genome.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
A human recombinant protein (amino acids Q416-T651) was used as the immunogen for the Beta Glucuronidase antibody.
Limitation:
This Beta Glucuronidase antibody is available for research use only.
Purity:
Affinity purified
Species Reactivity :
Human
Uniprot #:
P08236