CETP Antibody

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NSJ Bioreagents
Product Code: NSJ-RQ5582
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ5582-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the CETP antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Images

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IHC staining of FFPE human stomach cancer with CETP antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.
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IHC staining of FFPE human intestinal cancer with CETP antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.
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IHC staining of FFPE human breast cancer with CETP antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.
4 / 8
IHC staining of FFPE human placenta with CETP antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.
5 / 8
IHC staining of FFPE human ovarian cancer with CETP antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.
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IHC staining of FFPE human appendicitis tissue with CETP antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.
7 / 8
Western blot testing of 1) rat liver, 2) human ThP-1, 3) human Raji, 4) human U-87 MG and 5) mouse RAW246.7 lysate with CETP antibody. Expected molecular weight: 66-74 kDa depending on glycosylation level.
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Flow cytometry testing of human HepG2 cells with CETP antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= CETP antibody.
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IHC staining of FFPE human stomach cancer with CETP antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.
IHC staining of FFPE human intestinal cancer with CETP antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.
IHC staining of FFPE human breast cancer with CETP antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.
IHC staining of FFPE human placenta with CETP antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.
IHC staining of FFPE human ovarian cancer with CETP antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.
IHC staining of FFPE human appendicitis tissue with CETP antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.
Western blot testing of 1) rat liver, 2) human ThP-1, 3) human Raji, 4) human U-87 MG and 5) mouse RAW246.7 lysate with CETP antibody. Expected molecular weight: 66-74 kDa depending on glycosylation level.
Flow cytometry testing of human HepG2 cells with CETP antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= CETP antibody.

Documents

Further Information

Application Details :
Western blot: 0.25-0.5ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml,Flow cytometry: 1-3ug/million cells,Direct ELISA: 0.1-0.5ug/ml
Application Note:
Optimal dilution of the CETP antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide
Description:
CETP (Cholesteryl Ester Transfer Protein Plasma), is a plasma protein that facilitates the transport of cholesteryl esters and triglycerides between the lipoproteins. CETP is also known as lipid transfer protein I (Day et al., 1994). Sparkes et al. (1987) used a CETP probe against DNA from a human/mouse somatic cell hybrid panel to assign the CETP gene to chromosome 16. Because the role of CETP in atherosclerosis remained unclear, Okamoto et al. (2000) attempted to develop a potent, specific CETP inhibitor. One inhibitor, JTT-705, forms a disulfide bond with CETP and increases high density lipoprotein (HDL) cholesterol, decreases non-HDL cholesterol, and inhibits the progression of atherosclerosis in rabbits.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
A human recombinant protein (amino acids H77-K353) was used as the immunogen for the CETP antibody.
Limitation:
This CETP antibody is available for research use only.
Purity:
Affinity purified
Species Reactivity :
Human, Mouse, Rat
Uniprot #:
P11597