HLA-DRA Antibody / HLA-DR

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NSJ Bioreagents
Product Code: NSJ-RQ5585
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ5585-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the HLA-DRA antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Images

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Western blot testing of 1) human Raji, 2) rat spleen, 3) rat thymus, 4) mouse spleen and 5) mouse thymus lysate with HLA-DRA antibody. Expected molecular weight ~35 kDa.
2 / 7
Flow cytometry testing of human PBMC with HLA-DRA antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= HLA-DRA antibody.
3 / 7
Flow cytometry testing of human SW620 with HLA-DRA antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= HLA-DRA antibody.
4 / 7
IHC staining of FFPE human breast cancer with HLA-DRA antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.
5 / 7
IHC staining of FFPE human breast cancer with HLA-DRA antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.
6 / 7
IHC staining of FFPE mouse small intestine with HLA-DRA antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.
7 / 7
IHC staining of FFPE rat small intestine with HLA-DRA antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.
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Western blot testing of 1) human Raji, 2) rat spleen, 3) rat thymus, 4) mouse spleen and 5) mouse thymus lysate with HLA-DRA antibody. Expected molecular weight ~35 kDa.
Flow cytometry testing of human PBMC with HLA-DRA antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= HLA-DRA antibody.
Flow cytometry testing of human SW620 with HLA-DRA antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= HLA-DRA antibody.
IHC staining of FFPE human breast cancer with HLA-DRA antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.
IHC staining of FFPE human breast cancer with HLA-DRA antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.
IHC staining of FFPE mouse small intestine with HLA-DRA antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.
IHC staining of FFPE rat small intestine with HLA-DRA antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.

Documents

Further Information

Application Details :
Western blot: 0.25-0.5ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml,Flow cytometry: 1-3ug/million cells,Direct ELISA: 0.1-0.5ug/ml
Application Note:
Optimal dilution of the HLA-DRA antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide
Description:
HLA class II histocompatibility antigen, DR alpha chainis a protein that in humans is encoded by the HLA-DRA gene. It is mapped to 6p21.32. HLA-DRA is one of the HLA class II alpha chain paralogues. This class II molecule is a heterodimer consisting of an alpha and a beta chain, both anchored in the membrane. It plays a central role in the immune system by presenting peptides derived from extracellular proteins. Class II molecules are expressed in antigen presenting cells (APC: B lymphocytes, dendritic cells, macrophages). The alpha chain is approximately 33-35 kDa and its gene contains 5 exons. Exon 1 encodes the leader peptide, exons 2 and 3 encode the two extracellular domains, and exon 4 encodes the transmembrane domain and the cytoplasmic tail. DRA does not have polymorphisms in the peptide binding part and acts as the sole alpha chain for DRB1, DRB3, DRB4 and DRB5.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
A human recombinant protein (amino acids I26-L254) was used as the immunogen for the HLA-DRA antibody.
Limitation:
This HLA-DRA antibody is available for research use only.
Purity:
Affinity purified
Species Reactivity :
Human, Mouse, Rat
Uniprot #:
P01903