PCNA Antibody

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NSJ Bioreagents
Product Code: NSJ-RQ5594
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ5594-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the PCNA antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Images

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Flow cytometry testing of human 293T cells with PCNA antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= PCNA antibody.
2 / 6
IHC staining of FFPE human tonsil with PCNA antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.
3 / 6
IHC staining of FFPE human intestinal cancer with PCNA antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.
4 / 6
IHC staining of FFPE rat lymph tissue with PCNA antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.
5 / 6
Western blot testing of human Caco-2 cell lysate with PCNA antibody. Predicted molecular weight ~29 kDa, routinely observed at 29~36 kDa.
6 / 6
Western blot testing of 1) rat PC-12, 2) rat C6, 3) mouse NIH 3T3 and 4) mouse RAW246.7 cell lysate with PCNA antibody. Predicted molecular weight ~29 kDa, routinely observed at 29~36 kDa.
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Flow cytometry testing of human 293T cells with PCNA antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= PCNA antibody.
IHC staining of FFPE human tonsil with PCNA antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.
IHC staining of FFPE human intestinal cancer with PCNA antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.
IHC staining of FFPE rat lymph tissue with PCNA antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.
Western blot testing of human Caco-2 cell lysate with PCNA antibody. Predicted molecular weight ~29 kDa, routinely observed at 29~36 kDa.
Western blot testing of 1) rat PC-12, 2) rat C6, 3) mouse NIH 3T3 and 4) mouse RAW246.7 cell lysate with PCNA antibody. Predicted molecular weight ~29 kDa, routinely observed at 29~36 kDa.

Documents

Further Information

Application Details :
Western blot: 0.25-0.5ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml,Flow cytometry: 1-3ug/million cells,Direct ELISA: 0.1-0.5ug/ml
Application Note:
Optimal dilution of the PCNA antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide
Description:
Proliferating cell nuclear antigen (PCNA) is a DNA clamp that acts as a processivity factor for DNA polymerase delta in eukaryotic cells and is essential for replication. It is mapped to 20p12.3. The protein encoded by this gene is found in the nucleus and is a cofactor of DNA polymerase delta. The encoded protein acts as a homotrimer and helps increase the processivity of leading strand synthesis during DNA replication. In response to DNA damage, this protein is ubiquitinated and is involved in the RAD6-dependent DNA repair pathway. Two transcript variants encoding the same protein have been found for this gene. Pseudogenes of this gene have been described on chromosome 4 and on the X chromosome.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
A human recombinant protein (amino acids M1-S261) was used as the immunogen for the PCNA antibody.
Limitation:
This PCNA antibody is available for research use only.
Purity:
Affinity purified
Species Reactivity :
Human, Mouse, Rat
Uniprot #:
P12004