SUZ12 Antibody

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NSJ Bioreagents
Product Code: NSJ-RQ5598
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ5598-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the SUZ12 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Images

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IHC staining of FFPE human breast cancer with SUZ12 antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.
2 / 3
Flow cytometry testing of human ThP-1 cells with SUZ12 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= SUZ12 antibody.
3 / 3
Western blot testing of 1) human HL-60, 2) rat C6 and 3) mouse SP2/0 lysate with SUZ12 antibody. Expected molecular weight 83~95 kDa.
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IHC staining of FFPE human breast cancer with SUZ12 antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.
Flow cytometry testing of human ThP-1 cells with SUZ12 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= SUZ12 antibody.
Western blot testing of 1) human HL-60, 2) rat C6 and 3) mouse SP2/0 lysate with SUZ12 antibody. Expected molecular weight 83~95 kDa.

Documents

Further Information

Application Details :
Western blot: 0.25-0.5ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml,Flow cytometry: 1-3ug/million cells,Direct ELISA : 0.1-0.5ug/ml
Application Note:
Optimal dilution of the SUZ12 antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide
Description:
Polycomb protein SUZ12 is a protein that in humans is encoded by the SUZ12 gene. It is mapped to 17q11.2. This zinc finger gene has been identified at the breakpoints of a recurrent chromosomal translocation reported in endometrial stromal sarcoma. Recombination of these breakpoints results in the fusion of this gene and JAZF1. The protein encoded by this gene contains a zinc finger domain in the C terminus of the coding region.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
A human recombinant protein (amino acids K72-K483) was used as the immunogen for the SUZ12 antibody.
Limitation:
This SUZ12 antibody is available for research use only.
Purity:
Affinity purified
Species Reactivity :
Human, Mouse
Uniprot #:
Q15022