Osteoprotegerin Antibody / TNFRSF11B

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NSJ Bioreagents
Product Code: NSJ-RQ5604
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ5604-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the TNFRSF11B antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Images

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IHC staining of FFPE human placenta with TNFRSF11B antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.
2 / 13
IHC staining of FFPE human rectal cancer with TNFRSF11B antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.
3 / 13
IHC staining of FFPE human oesophagus squama with TNFRSF11B antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.
4 / 13
IHC staining of FFPE human appendicitis tissue with TNFRSF11B antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.
5 / 13
IHC staining of FFPE human endometrial carcinoma with TNFRSF11B antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.
6 / 13
IHC staining of FFPE human glioma with TNFRSF11B antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.
7 / 13
IHC staining of FFPE human tonsil with TNFRSF11B antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.
8 / 13
IHC staining of FFPE human lung cancer with TNFRSF11B antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.
9 / 13
IHC staining of FFPE human liver cancer with TNFRSF11B antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.
10 / 13
IHC staining of FFPE human breast cancer with TNFRSF11B antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.
11 / 13
IHC staining of FFPE mouse brain with TNFRSF11B antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.
12 / 13
IHC staining of FFPE rat brain with TNFRSF11B antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.
13 / 13
Flow cytometry testing of human U-2 OS cells with TNFRSF11B antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= TNFRSF11B antibody.
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IHC staining of FFPE human placenta with TNFRSF11B antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.
IHC staining of FFPE human rectal cancer with TNFRSF11B antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.
IHC staining of FFPE human oesophagus squama with TNFRSF11B antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.
IHC staining of FFPE human appendicitis tissue with TNFRSF11B antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.
IHC staining of FFPE human endometrial carcinoma with TNFRSF11B antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.
IHC staining of FFPE human glioma with TNFRSF11B antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.
IHC staining of FFPE human tonsil with TNFRSF11B antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.
IHC staining of FFPE human lung cancer with TNFRSF11B antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.
IHC staining of FFPE human liver cancer with TNFRSF11B antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.
IHC staining of FFPE human breast cancer with TNFRSF11B antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.
IHC staining of FFPE mouse brain with TNFRSF11B antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.
IHC staining of FFPE rat brain with TNFRSF11B antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.
Flow cytometry testing of human U-2 OS cells with TNFRSF11B antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= TNFRSF11B antibody.

Documents

Further Information

Application Details :
Western blot: 0.25-0.5ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml,Flow cytometry: 1-3ug/million cells,Direct ELISA: 0.1-0.5ug/ml
Application Note:
Optimal dilution of the TNFRSF11B antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide
Description:
Tumor necrosis factor receptor superfamily member 11B (TNFRSF11B), also known as OPG, is a protein that in humans is encoded by the TNFRSF11B gene. OPG is a cytokine receptor, and a member of the tumor necrosis factor (TNF) receptor superfamily. By analysis of radiation hybrids, TNFRSF11B gene was mapped to chromosome 8q24. OPG is a decoy receptor for the receptor activator of nuclear factor kappa B ligand (RANKL). By binding RANKL, OPG inhibits nuclear kappa B (NF-?B) which is a central and rapid acting transcription factor for immune-related genes, and a key regulator of inflammation, innate immunity, and cell survival and differentiation. OPG binding to RANKL on osteoblast/stromal cells, blocks the RANKL-RANK ligand interaction between osteoblast/stromal cells and ostepclast precursors.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
A human recombinant protein (amino acids Q247-R296) was used as the immunogen for the TNFRSF11B antibody.
Limitation:
This TNFRSF11B antibody is available for research use only.
Purity:
Affinity purified
Species Reactivity :
Human, Mouse, Rat
Uniprot #:
O00300