MVP Antibody / Major Vault Protein

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NSJ Bioreagents
Product Code: NSJ-RQ5611
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ5611-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Mouse
Antibody Isotype: Mouse IgG2a
Antibody Clonality: Monoclonal
Antibody Clone: 8B12
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the MVP antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Images

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IHC staining of FFPE human lung cancer with MVP antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.
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IHC staining of FFPE human lung cancer with MVP antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.
3 / 9
IHC staining of FFPE human breast cancer with MVP antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.
4 / 9
IHC staining of FFPE human colon cancer with MVP antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.
5 / 9
IHC staining of FFPE mouse intestine with MVP antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.
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IHC staining of FFPE rat intestine with MVP antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.
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Western blot testing of human 1) A549, 2) U-2 OS, 3) HepG2, 4) ThP-1, 5) HeLa, 6) SW620, 7) rat RH35 and 8) mouse RAW264.7 lysate with MVP antibody. Observed molecular weight: 104~110 kDa.
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Flow cytometry testing of human A431 cells with MVP antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= MVP antibody.
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Flow cytometry testing of human A549 cells with MVP antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= MVP antibody.
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IHC staining of FFPE human lung cancer with MVP antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.
IHC staining of FFPE human lung cancer with MVP antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.
IHC staining of FFPE human breast cancer with MVP antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.
IHC staining of FFPE human colon cancer with MVP antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.
IHC staining of FFPE mouse intestine with MVP antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.
IHC staining of FFPE rat intestine with MVP antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.
Western blot testing of human 1) A549, 2) U-2 OS, 3) HepG2, 4) ThP-1, 5) HeLa, 6) SW620, 7) rat RH35 and 8) mouse RAW264.7 lysate with MVP antibody. Observed molecular weight: 104~110 kDa.
Flow cytometry testing of human A431 cells with MVP antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= MVP antibody.
Flow cytometry testing of human A549 cells with MVP antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= MVP antibody.

Documents

Further Information

Application Details :
Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml,Flow cytometry: 1-3ug/million cells
Application Note:
Optimal dilution of the MVP antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide
Description:
Major vault protein is a protein that in humans is encoded by the MVP gene. This gene encodes the major component of the vault complex. Vaults are multi-subunit ribonucleoprotein structures that may be involved in nucleo-cytoplasmic transport. The encoded protein may play a role in multiple cellular processes by regulating the MAP kinase, JAK/STAT and phosphoinositide 3-kinase/Akt signaling pathways. The encoded protein also plays a role in multidrug resistance, and expression of this gene may be a prognostic marker for several types of cancer. Alternatively spliced transcript variants have been observed for this gene.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
A human recombinant protein (amino acids A2-H259) was used as the immunogen for the MVP antibody.
Limitation:
This MVP antibody is available for research use only.
Purity:
Affinity purified
Species Reactivity :
Human, Mouse, Rat
Uniprot #:
Q14764