Histone H1.0 Antibody / H1F0

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NSJ Bioreagents
Product Code: NSJ-RQ5613
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ5613-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the Histone H1.0 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Images

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IHC staining of FFPE human breast cancer with Histone H1.0 antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.
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IHC staining of FFPE human breast cancer with Histone H1.0 antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.
3 / 12
IHC staining of FFPE human lung cancer with Histone H1.0 antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.
4 / 12
IHC staining of FFPE mouse liver with Histone H1.0 antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.
5 / 12
IHC staining of FFPE mouse liver with Histone H1.0 antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.
6 / 12
IHC staining of FFPE mouse small intestine with Histone H1.0 antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.
7 / 12
IHC staining of FFPE mouse small intestine with Histone H1.0 antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.
8 / 12
IHC staining of FFPE rat liver with Histone H1.0 antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.
9 / 12
IHC staining of FFPE rat liver with Histone H1.0 antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.
10 / 12
IHC staining of FFPE rat small intestine with Histone H1.0 antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.
11 / 12
Western blot testing of human 1) U-2 OS, 2) PC-3, 3) Caco-2 and 4) HepG2 cell lysate with Histone H1.0 antibody. Predicted molecular weight ~20 kDa but can be observed at 27-30 kDa.
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Western blot testing of mouse 1) lung and 2) NIT 3T3 lysate with Histone H1.0 antibody. Predicted molecular weight ~20 kDa but can be observed at 27-30 kDa.
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IHC staining of FFPE human breast cancer with Histone H1.0 antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.
IHC staining of FFPE human breast cancer with Histone H1.0 antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.
IHC staining of FFPE human lung cancer with Histone H1.0 antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.
IHC staining of FFPE mouse liver with Histone H1.0 antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.
IHC staining of FFPE mouse liver with Histone H1.0 antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.
IHC staining of FFPE mouse small intestine with Histone H1.0 antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.
IHC staining of FFPE mouse small intestine with Histone H1.0 antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.
IHC staining of FFPE rat liver with Histone H1.0 antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.
IHC staining of FFPE rat liver with Histone H1.0 antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.
IHC staining of FFPE rat small intestine with Histone H1.0 antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.
Western blot testing of human 1) U-2 OS, 2) PC-3, 3) Caco-2 and 4) HepG2 cell lysate with Histone H1.0 antibody. Predicted molecular weight ~20 kDa but can be observed at 27-30 kDa.
Western blot testing of mouse 1) lung and 2) NIT 3T3 lysate with Histone H1.0 antibody. Predicted molecular weight ~20 kDa but can be observed at 27-30 kDa.

Documents

Further Information

Application Details :
Western blot: 0.25-0.5ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml,Direct ELISA: 0.1-0.5ug/ml
Application Note:
Optimal dilution of the Histone H1.0 antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide
Description:
H1 histone family, member 0 is a member of the histone family of nuclear proteins which are a component of chromatin. In humans, this protein is encoded by the H1F0 gene. It is mapped to 22q13.1. Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-independent histone that is a member of the histone H1 family.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
A human recombinant protein (amino acids K20-K159) was used as the immunogen for the Histone H1.0 antibody.
Limitation:
This Histone H1.0 antibody is available for research use only.
Localization:
Nuclear
Purity:
Affinity purified
Species Reactivity :
Human, Mouse, Rat
Uniprot #:
P07305